publication . Article . 2010

Alternatively spliced N-terminal exons in tropomyosin isoforms do not act as autonomous targeting signals

Claire Martin; Galina Schevzov; Peter W. Gunning;
Open Access
  • Published: 01 May 2010 Journal: Journal of Structural Biology, volume 170, pages 286-293 (issn: 1047-8477, Copyright policy)
  • Publisher: Elsevier BV
Abstract
Tropomyosin (Tm) polymerises head-to-tail to form a continuous polymer located in the major groove of the actin filament. Multiple Tm isoforms are generated by alternative splicing of four genes, and individual isoforms show specific localisation patterns in many cell types, and can have differing effects on the actin cytoskeleton. Fluorescently-tagged Tm isoforms and mutants were expressed in C2C12 cells to investigate the mechanisms of alternative localisation of high molecular weight (HMW) and low molecular weight (LMW) Tms. Fluorescently-tagged Tm constructs show similar localisation to endogenous Tms as observed by antibodies, with the HMW Tm3 relatively di...
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free text keywords: Structural Biology, Arp2/3 complex, biology.protein, biology, Cytoskeleton, Gene isoform, Alternative splicing, Actin cytoskeleton, Cell biology, Fluorescence recovery after photobleaching, Actin, Tropomyosin
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