publication . Article . 2004

Peptide and protein analysis by electrospray ionization-mass spectrometry and capillary electrophoresis-mass spectrometry

Joseph A. Loo; Harold R. Udseth; Richard D. Smith;
Open Access
  • Published: 08 Dec 2004
Abstract
The extension of mass spectrometry to high molecular weight biopolymers based upon electrospray ionization and the on-line combination with capillary electrophoresis is described. Electrospray ionization produces gas-phase intact multiply charged molecular ions of biomolecules from highly charged liquid droplets by a high electric field. For high molecular weight substances electrospray ionization results in a characteristic bell-shaped distribution of multiply charged ions, with each adjacent major peak in the spectrum differing by one charge. Multiply charged molecular ions of proteins with molecular weights greater than 130,000 have been observed with a quadr...
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free text keywords: Biophysics, Cell Biology, Biochemistry, Molecular Biology, Chemistry, Mass spectrometry, Chromatography, Atmospheric-pressure chemical ionization, Sample preparation in mass spectrometry, Capillary electrophoresis–mass spectrometry, Direct electron ionization liquid chromatography–mass spectrometry interface, Analytical chemistry, Protein mass spectrometry, Extractive electrospray ionization, Electrospray ionization
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