publication . Article . 2018

Peptidomic approach identifies cruzioseptins, a new family of potent antimicrobial peptides in the splendid leaf frog, Cruziohyla calcarifer

Carolina Proaño-Bolaños; Mei Zhou; Lei Wang; Luis A. Coloma; Tianbao Chen; Chris Shaw;
Open Access English
  • Published: 02 Dec 2018
  • Publisher: Elsevier
  • Country: Bangladesh
Abstract
Abstract Phyllomedusine frogs are an extraordinary source of biologically active peptides. At least 8 families of antimicrobial peptides have been reported in this frog clade, the dermaseptins being the most diverse. By a peptidomic approach, integrating molecular cloning, Edman degradation sequencing and tandem mass spectrometry, a new family of antimicrobial peptides has been identified in Cruziohyla calcarifer. These 15 novel antimicrobial peptides of 20–32 residues in length are named cruzioseptins. They are characterized by having a unique shared N-terminal sequence GFLD– and the sequence motifs –VALGAVSK– or –GKAAL(N/G/S) (V/A)V– in the middle of the pepti...
Subjects
free text keywords: Antimicrobial peptides, Cruzioseptins, Molecular cloning, Peptidomic, Skin secretions, Cruzioseptins; antimicrobial peptides; peptidomic; molecular cloning; skin secretions; tandem massspectrometry, Edman degradation, Peptide, chemistry.chemical_classification, chemistry, Haemolysis, Antimicrobial, Gram-negative bacteria, biology.organism_classification, biology, Biochemistry, Cruziohyla calcarifer
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publication . Article . 2018

Peptidomic approach identifies cruzioseptins, a new family of potent antimicrobial peptides in the splendid leaf frog, Cruziohyla calcarifer

Carolina Proaño-Bolaños; Mei Zhou; Lei Wang; Luis A. Coloma; Tianbao Chen; Chris Shaw;