publication . Doctoral thesis . 1990

Erwinia carotovora extracellular proteases: characterization and role in soft rot

Kyöstiö, Sirkka R. M.;
Open Access
  • Published: 03 Dec 1990
  • Publisher: Virginia Tech
  • Country: United States
<u>Erwinia carotovora</u> subsp. <u>carotovora</u> (Ecc) strain EC14, a Gram-negative bacterium, causes soft rot on several crops, including potato. Maceration of potato tuber tissue is caused by secreted pectolytic enzymes. Other cell-degrading enzymes may also have roles in pathogenesis, including cellulases, phospholipases, and protease(s). The objectives of this research were to (1) characterize Ecc extracellular protease (Prt) and (2) elucidate its role in potato soft rot. A gene encoding a Prt, <u>prt</u>1, was cloned from cosmid pCA7 containing Ecc genomic DNA into plasmid pSK1. <u>Escherichia coli</u> transformed with pSK1 or pSK23, a subclone of pSK1, p...
Medical Subject Headings: food and beverages
free text keywords: Bacterial soft rot of potato., LD5655.V856 1990.K967
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Doctoral thesis . 1990
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