publication . Thesis . 2001

Isolation and Characterization of D-Myo-Inositol-3-Phosphate Synthase Gene Family Members in Soybean

Good, Laura Lee;
Open Access
  • Published: 10 Aug 2001
  • Publisher: Virginia Tech
  • Country: United States
Abstract
The objective of this research was to isolate genes encoding isoforms of the enzyme D-myo-inositol 3-phosphate synthase (MIPS, E.C. 5.5.1.4) from soybean and to characterize their expression, especially with respect to their involvement in phytic acid biosynthesis. A MIPS-homologous cDNA, designated GmMIPS1, was isolated via PCR using total RNA from developing seeds. Southern blot analysis and examination of MIPS-homologous soybean EST sequences suggested that GmMIPS1 is part of a multigene family of at least four similar members. The sequences of promoter and genomic regions of GmMIPS1 and GmMIPS2 revealed a high degree of sequence conservation. Northern and we...
Subjects
Medical Subject Headings: food and beverages
free text keywords: MIPS, myo-inositol phosphate synthase, Glycine max, phytic acid, soybean seed development
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Thesis . 2001
Provider: VTechWorks
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