publication . Thesis . 2003

Analysis of Biogenic Amines by GC/FID and GC/MS

Nakovich, Laura;
Open Access
  • Published: 16 Jul 2003
  • Publisher: Virginia Tech
  • Country: United States
Low levels of biogenic amines occur naturally, but high levels (FDA sets 50 ppm of histamine in fish as the maximum allowable level) can lead to scombroid poisoning. Amines in general are difficult to analyze by Gas Chromatography (GC) due to their lack of volatility and their interaction with the GC column, often leading to significant tailing and poor reproducibility. Biogenic amines need to be derivatized before both GC and HPLC analyses. The objective of this research was to develop a relatively fast, reproducible method to derivatize and quantitate biogenic amines in fish at trace levels using GC/FID. The derivatizing reagent used in the experiments was pro...
free text keywords: Gas Chromatography, Mass Spectroscopy, Propyl Chloroformate Derivatives, Biogenic Amines
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Thesis . 2003
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