publication . Bachelor thesis . 2013

Analysis of metabolome effects of hop (H. lupulus) transcription factors in heterologous of Petunia hybrida

MORAVCOVÁ, Vendula;
Restricted Czech
  • Published: 01 Jan 2013
  • Country: Czech Republic
Abstract
Plant transformation is now a key research in plant biology, and also is a unique practical tool in the improvement of varieties of cultivated plants. The aim of this work was to obtain transgenic plants Petunia x hybrida containing transgene nptII (kanamycin resistance gene) using the indirect transformation by Agrobacterium tumefaciens. In terms of in-vivo was grown from seeds experimental material in the form of plant Petunia x hybrida cv. Andrea. For the experiments three different constructs HLWD40 3278-80 and HLbHLH 3577 and HLbHLH 3677 GFP were used. The leaf explants from Petunia plants were prepared. Explants were transferred using tweezers into the cru...
Subjects
Medical Subject Headings: food and beveragesfungi
free text keywords: Agrobacterium tumefaciens; PCR; Petunia x hybrida; Transformace; Transformation
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