publication . Article . 2021

Thrombin regulates tissue factor and thrombomodulin mRNA levels and activities in human saphenous vein endothelial cells by distinct mechanisms

K. Bartha; C. Brisson; G. Archipoff; C. de la Salle; F. Lanza; J.P. Cazenave; A. Beretz;
Open Access
  • Published: 05 Jan 2021
  • Country: France
The effects of thrombin, D-phenylalanyl-L-propyl-L-arginine chloromethyl ketone (PPACK)-inhibited thrombin, and thrombin receptor agonist peptide, SFLLRNPNDKYEPF (SFLL, a portion of the receptor unmasked after thrombin cleavage), on the expression of tissue factor (TF) and thrombomodulin by human saphenous vein endothelial cells (HSVECs) in culture were studied. Unstimulated cells contained very low amounts of TF mRNA as measured by the reverse transcriptase-PCR method. Thrombin treatment increased TF mRNA to 8.0 +/- 1.9 (n = 3) times the control level. The increase was detectable within 2 h and declined to near basal level by 6 h. Induction of TF mRNA was not b...
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Medical Subject Headings: cardiovascular systemcirculatory and respiratory physiology
free text keywords: Non-U.S. Gov't Saphenous Vein Thrombin/*pharmacology Thromboplastin/biosynthesis/*genetics, Amino Acid Chloromethyl Ketones/pharmacology Amino Acid Sequence Base Sequence Cells, Cultured Comparative Study Cycloheximide/pharmacology Endothelium, Vascular/drug effects/*physiology Humans Immunoenzyme Techniques Kinetics Molecular Sequence Data Oligodeoxyribonucleotides Peptides/*pharmacology Polymerase Chain Reaction/methods RNA/genetics/isolation and purification RNA, Messenger/*metabolism Receptors, Cell Surface/biosynthesis/drug effects/*genetics/physiology Receptors, Thrombin Research Support, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences, Cell Biology, Molecular Biology, Biochemistry, Molecular biology, Immunoelectron microscopy, Cycloheximide, chemistry.chemical_compound, chemistry, Biology, Platelet, Tissue factor, Thrombomodulin, Receptor, Thrombin, medicine.drug, medicine, Thrombin receptor
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