publication . Article . 2002

Reciprocal translocations in breast tumor cell lines: Cloning of a t(3;20) that targets the FHIT gene

Popovici, C.; Basset, C.; Bertucci, F.; Orsetti, A.; Adelaide, J.; Mozziconacci, M. J.; Conte, N.; Murati, A.; Ginestier, C.; Charafe-Jauffret, E.; ...
English
  • Published: 01 Jan 2002
  • Publisher: HAL CCSD
  • Country: France
Abstract
All molecular alterations that lead to breast cancer are not precisely known. We are evaluating the frequency and consequences of reciprocal translocations in breast cancer. We surveyed 15 mammary cell lines by multicolor fluorescence in situ hybridization (M-FISH). We identified nine apparently reciprocal translocations. Using mBanding FISH and FISH with selected YAC clones, we identified the breakpoints for four of them, and cloned the t(3;20)(p 14;p 11) found in the BrCa-MZ-02 cell line. We found that the breakpoint targets the potential tumor-suppressor gene FHIT (fragile histidine triad) in the FRA3B region; it is accompanied by homozygous deletion of exon ...
Subjects
free text keywords: comparative genomic hybridization clonal chromosome-abnormalities molecular cytogenetic analysis DNA amplification gamma-heregulin deficient mice cancer carcinomas expression region, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
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