publication . Article . Other literature type . 2016

Diagnosis of aerobic vaginitis by quantitative real-time PCR.

Ta, Rumyantseva; Bellen G; Ya, Savochkina; Ae, Guschin; Gilbert Donders;
  • Published: 18 Jan 2016 Journal: Archives of Gynecology and Obstetrics, volume 294, pages 109-114 (issn: 0932-0067, eissn: 1432-0711, Copyright policy)
  • Publisher: Springer Science and Business Media LLC
  • Country: Belgium
Abstract: Purpose To evaluate a real-time PCR-based technique to quantify bacteria associated with aerobic vaginitis (AV) as a potential test. Methods Vaginal samples from 100 women were tested by wet-mount microscopy, gram stain and quantitative real-time PCR targeting Enterobacteriacea, Staphylococcus spp., Streptococcus spp., Enterococcus spp., Escherichia coli, Streptococcus agalactiae, S. aureus; Lactobacillus spp. AV diagnosis obtained by wet-mount microscopy was used as reference. Results Some level of AV was diagnosed in 23 (23.7 %) cases. Various concentrations of Enterobacteriacea, Staphylococcus spp., Streptococcus spp. were detected an all patients. ...
free text keywords: Human medicine, Obstetrics and Gynaecology, General Medicine, Aerobic vaginitis, Microbiology, Polymerase chain reaction, law.invention, law, Vaginitis, medicine.disease, medicine, Quantitative Real Time PCR, Bacterial vaginosis, business.industry, business, Real-time polymerase chain reaction
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