publication . Article . 2018

A calibrated diversity assay for nucleic acid libraries using DiStRO—a Diversity Standard of Random Oligonucleotides

Schütze, Tatjana; Arndt, Peter F.; Menger, Marcus; Wochner, Aniela; Vingron, Martin; Erdmann, Volker A.; Lehrach, Hans; Kaps, Christian; Glökler, Jörn;
Open Access
  • Published: 13 Nov 2018 Journal: Nucleic Acids Research, volume 38, pages e23-e23 (issn: 0305-1048, eissn: 1362-4962, Copyright policy)
  • Publisher: Oxford University Press (OUP)
We have determined diversities exceeding 10(12) different sequences in an annealing and melting assay using synthetic randomized oligonucleotides as a standard. For such high diversities, the annealing kinetics differ from those observed for low diversities, favouring the remelting curve after annealing as the best indicator of complexity. Direct comparisons of nucleic acid pools obtained from an aptamer selection demonstrate that even highly complex populations can be evaluated by using DiStRO, without the need of complicated calculations.
Medical Subject Headings: human activitiesrespiratory system
free text keywords: Nucleic acid thermodynamics, SELEX Aptamer Technique, Molecular biology, Oligonucleotide, Genetics, Biology, Reference standards, Nucleic Acid Denaturation, Nucleic acid, Aptamer, Methods Online
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