publication . Article . 2012

Agrobacterium rhizogenes-dependent production of transformed roots from foliar explants of pepper (Capsicum annuum): a new and efficient tool for functional analysis of genes

Aarrouf, Jawad; Castro Quezada, Patricio; Mallard, Simon; Caromel, Bernard; Lizzi, Y; Lefebvre, Véronique;
Open Access
  • Published: 01 Feb 2012 Journal: Plant Cell Reports, volume 31, pages 391-401 (issn: 0721-7714, eissn: 1432-203X, Copyright policy)
  • Publisher: Springer Science and Business Media LLC
Abstract
International audience; Pepper is known to be a recalcitrant species to genetic transformation via Agrobacterium tumefaciens. A. rhizogenes-mediated transformation offers an alternative and rapid possibility to study gene functions in roots. In our study, we developed a new and efficient system for A. rhizogenes transformation of the cultivated species Capsicum annuum. Hypocotyls and foliar organs (true leaves and cotyledons) of Yolo Wonder (YW) and Criollo de Morelos 334 (CM334) pepper cultivars were inoculated with the two constructs pBIN-gus and pHKN29-gfp of A. rhizogenes strain A4RS. Foliar explants of both pepper genotypes infected by A4RS-pBIN-gus or A4RS...
Subjects
Medical Subject Headings: food and beveragesfungi
free text keywords: Agronomy and Crop Science, Plant Science, General Medicine, Phytophthora capsici, biology.organism_classification, biology, Agrobacterium, Agrobacterium tumefaciens, Hypocotyl, Cotyledon, food.ingredient, food, Transformation (genetics), Transformation efficiency, Botany, Pepper, AGROBACTERIUM RHIZOGENES, BETA GLUCURONIDASE, GREEN FLUORESCENT PROTEIN, [SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Génétique des plantes, Plants genetics, AGROBACTERIUM RHIZOGENES;PHYTOPHTHORA CAPSICI;BETA GLUCURONIDASE;TRANSFORMATION EFFICIENCY;GREEN FLUORESCENT PROTEIN, capsicum annuum, poivron, plante légumière, bactérie, gène de résistance, racine, transformation génétique, pcr, résistance aux maladies
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publication . Article . 2012

Agrobacterium rhizogenes-dependent production of transformed roots from foliar explants of pepper (Capsicum annuum): a new and efficient tool for functional analysis of genes

Aarrouf, Jawad; Castro Quezada, Patricio; Mallard, Simon; Caromel, Bernard; Lizzi, Y; Lefebvre, Véronique;