publication . Article . 2015

Comparison of microcalorimetry and haze formation to quantify the association of B-type procyanidins to poly-l-proline and bovine serum albumin

Watrelot, Aude; Renard, Catherine; Le Bourvellec, Carine;
Open Access English
  • Published: 01 Jan 2015
  • Publisher: HAL CCSD
Abstract
Abstract Though many different methods have been applied to protein-tannin interactions, divergent results are often reported. To better understand the origin of these differences, we compare here haze/aggregates formation and thermodynamic parameters occurring for protein-procyanidin interactions. Proteins well referenced for interaction with polyphenols, namely a polypeptide of extended structure that resembles salivary proteins (poly- l -proline (PLP) and a standard globular protein (bovine serum albumin (BSA)) were used. Flavan-3-ols of three degrees of polymerization were tested, (-)-epicatechin (EPI), a dimer (DP2) and a procyanidin (DP8). The interactions...
Subjects
Medical Subject Headings: lipids (amino acids, peptides, and proteins)
free text keywords: Polyphenol, Affinity, Precipitate, ITC, Protein, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], Polyphenol;Protein;Affinity;Precipitate;ITC, malus domestica borkh, procyanidine, protéine, agrégat, microcalorimétrie, spectrophotométrie, chromatographie hplc, Dimer, chemistry.chemical_compound, chemistry, Isothermal microcalorimetry, Polymerization, Bovine serum albumin, biology.protein, biology, Biochemistry, Proline, Globular protein, chemistry.chemical_classification, Isothermal titration calorimetry, Chromatography
Funded by
EC| DREAM
Project
DREAM
Design and development of REAlistic food Models with well-characterised micro- and macro-structure and composition
  • Funder: European Commission (EC)
  • Project Code: 222654
  • Funding stream: FP7 | SP1 | KBBE
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The authors thank Dr. K. Kurtural for careful rereading of English et u expression. The research leading to these results has received V /A funding from the European Community's Seventh Framework r Programme (FP7/2007-2013) under the grant agreement n FP7- u e 222 654-DREAM.

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