publication . Article . 2011

Improved sensitivity using liquid chromatography mass spectrometry (LC-MS) for detection of propyl chloroformate derivatised β-N-methylamino-L-alanine (BMAA) in cyanobacteria

M Esterhuizen-Londt; S Downing; TG Downing;
Open Access English
  • Published: 01 Apr 2011
  • Publisher: Water Research Commission (WRC)
Abstract
β-N-methylamino-L-alanine (BMAA) is a difficult molecule to detect, primarily due to its presence in low concentrations in complex matrices. This has resulted in contradictory reports on the presence of BMAA in cyanobacteria. We report improved sensitivity of detection using propyl chloroformate derivatisation, liquid chromatographic (LC) separation, and single quadrupole mass spectrometry (MS) detection. Triple quadrupole mass spectrometry (MS/MS) was used to confirm the identity of BMAA in cyanobacteria based on product ions. We show a 10-fold increase in sensitivity with the LC-MS method compared to the previously published gas chromatography mass spectrometr...
Subjects
free text keywords: β-N-methylamino-L-alanine, BMAA, LC-MS, LC-MS/MS, Waste Management and Disposal, Applied Microbiology and Biotechnology, Management, Monitoring, Policy and Law, Water Science and Technology, Cyanobacteria, biology.organism_classification, biology, Alanine, Gas chromatography–mass spectrometry, Amino acid, chemistry.chemical_classification, chemistry, Mass spectrometry, Gas chromatography, Chromatography, Molecule, Liquid chromatography–mass spectrometry
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publication . Article . 2011

Improved sensitivity using liquid chromatography mass spectrometry (LC-MS) for detection of propyl chloroformate derivatised β-N-methylamino-L-alanine (BMAA) in cyanobacteria

M Esterhuizen-Londt; S Downing; TG Downing;