publication . Article . 2017

Sperm DNA damage in relation to lipid peroxidation following freezing-thawing of boar semen

Leyland Fraser;
Open Access English
  • Published: 01 Jan 2017
  • Publisher: The South African Society for Animal Science (SASAS)
Abstract
This study investigated the relationships between lipid peroxidation (LPO) and sperm DNA damage following freezing-thawing of boar semen in different extenders. The comet assay was used to measure the extent of sperm DNA damage in a cryoprotectant-free extender or in cryoprotectant-based extenders after single and repeated freezing and thawing. As well as an analysis of sperm motion characteristics, mitochondrial function, membrane integrity, and lipid peroxidation (LPO) were assessed simultaneously with the measurements of sperm DNA damage. Consistent positive significant correlations were found between sperm DNA damage and LPO after freezing-thawing. Comet ass...
Subjects
Medical Subject Headings: urogenital systemendocrine systemreproductive and urinary physiology
free text keywords: Comet assay measurements, cryopreservation, extenders, spermatozoa
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