publication . Doctoral thesis . Master thesis . 2012

The sylvatic and synanthropic cycles of Echinococcus SPP., Taenia SPP. and Toxocara SPP. in Portugal : coprologic and molecular diagnosis in canids

Guerra, Diogo Ribeiro Almeida;
Open Access
  • Published: 02 May 2012
  • Country: Portugal
Abstract
Dissertação de Mestrado Integrado em Medicina Veterinária Echinococcus spp., Taenia spp. and Toxocara spp. are important parasites of domestic and wild canids and neglected zoonotic helminths. Despite their relevance in Public Health, little is known about their prevalence in Portugal. An epidemiological study was conducted to clarify the role of canids in the sylvatic and synanthropic cycles of these pathogens in our country. Fecal samples from dog (n = 51), red fox (n = 62) and Iberian wolf (n = 68) were collected from two regions. Toxocara spp. and taeniid eggs were isolated through a Sieving-flotation technique. Species identification in taeniids was made us...
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Subjects
free text keywords: Canids, Echinococcus, Epidemiology, Portugal, Taenia, Toxocara, Canídeos, Epidemiologia
Related Organizations
217 references, page 1 of 15

Figure 1 - Morphology of different stages of Echinococcus granulosus. (Original illustrations) ....... 6

Figure 2 - Morphology of different stages of Taenia spp. (Original illustrations)............................ 23

Figure 3 - Taenia species infecting Iberian wolf in Northwestern Iberian Peninsula (Original) ...... 27

Figure 4 - Prevalence of Taeniid eggs in dog's fecal samples collected from public places.

(Original map) ............................................................................................................................... 29

Figure 5 - Morphology of different stages of T. canis and T. cati. (Original illustrations) ............... 32

Figure 6 - Prevalence of Toxocara sp. eggs in dog's fecal samples collected from public places.

(Original map) ............................................................................................................................... 37

Figure 7 - Areas of study and geographical distribution of fecal samples. (Original map) ............. 44

Figure 8 - Decision Tree for samples analysis.............................................................................. 47

Figure 9 - A 21-μm sieve for egg isolation. ................................................................................... 48

Figure 10 - Taeniid eggs in a wolf fecal sample positive for T. hydatigena. (Original pictures)...... 52

Figure 11 - Example of Multiplex-PCR results of two samples (Original picture)........................... 52

Figure 12 - Approximate geographic location of samples positive for taeniid species................... 53

Figure 13 - (a) unembryonated (b) embryonated and (c) larvated Toxocara spp. eggs recovered

217 references, page 1 of 15
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