Investigations of CHO 1-15 and Silk Gland Cell Line Development
Other literature type
Robinson, Susan Kathleen
- Publisher: DigitalCommons@USU
cell line development | CHO-1-15 | silk gland cell
The BioProcessing Demonstration and Training Laboratory was established with a collaboration between Utah State University and Thermo Fisher- Scientific, Inc. This lab was developed into a fully functioning tissue culture facility. Demonstration of tissue culture procedures were necessary for the lab to be industry-fully qualified. The CHO 1-15 cell line protocols were optimized by establishing conditions for reproducibility in shaker flasks and bioreactors (2 to 250 L capacity). CHO 1-15 is the cell line of choice for protein production in the bio-manufacturing industry. Oxidative stress is a problem in the industry because it can cause a decrease in protein production. Mesobiliverdin IXα and biliverdin IXα possess antioxidant capabilities. The effects of the antioxidant nature of these compounds were tested on the CHO 1-15 cell line. Cell cultures from silkworm and spider silk producing cells were also pursued. Methods to produce a primary cell line from spider silk gland cells were developed. Cell lines from spider and silkworm silk producing glands appeared to have the capacity to secrete full-length native proteins ranging in size from 200 to 500 kDa, and possibly larger.