publication . Article . 1981

Proliferación de las células no neuronales del ganglio ciliar en cultivos in vitro

Padilla-Alvarez, F.; Alcaín Tejada, F.J.; Bustos Ruiz, M.;
Open Access Spanish; Castilian
  • Published: 01 Jan 1981
  • Publisher: Universidad de Córdoba, Servicio de Publicaciones
  • Country: Spain
In our investigations, non-neuron cells from ciliary ganglion of chick embryos (8 days-old), were cultured on tissue culture plastic dis¬hes, using as culture media heart-conditioned medium (HCM), HCM supple¬mented "with glucose (HCMC) and with phytohemagglutinin (PHA) added to HCM (HCMPHA). The low rate of proliferation observed in these ceil.s in ovo, per¬sists when the cells are cultured in vitro. The higest index of ,proliferation is obtained by using HCM as cultu re medium. We observed that PHA prevents mitosis and produces cell vacuo-les. The effect of glucose encourages cell adhesion, but in decreases the mitosis index. En nuestras investigaciones, cultiv...
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free text keywords: Ganglios ciliares, Células no neuronales, PHA, MCC
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