publication . Article . Other literature type . 2017

BDNF gene delivery mediated by neuron-targeted nanoparticles is neuroprotective in peripheral nerve injury

Lopes, CDF; Gonçalves, NP; Gomes, CP; Saraiva, MJ; Pêgo, AP;
Open Access English
  • Published: 01 Mar 2017
  • Publisher: Elsevier
  • Country: Portugal
Neuron-targeted gene delivery is a promising strategy to treat peripheral neuropathies. Here we propose the use of polymeric nanoparticles based on thiolated trimethyl chitosan (TMCSH) to mediate targeted gene delivery to peripheral neurons upon a peripheral and minimally invasive intramuscular administration. Nanoparticles were grafted with the non-toxic carboxylic fragment of the tetanus neurotoxin (HC) to allow neuron targeting and were explored to deliver a plasmid DNA encoding for the brain-derived neurotrophic factor (BDNF) in a peripheral nerve injury model. The TMCSH-HC/BDNF nanoparticle treatment promoted the release and significant expression of BDNF i...
free text keywords: Brain-derived neurotrophic factor, Gene therapy, Neuron-targeted nanoparticles, Neuropathies, Neuroprotection, Trimethyl chitosan, Animals, Brain-Derived Neurotrophic Factor/genetics, Female, Genetic Therapy/methods, Mice, Mice Inbred BALB C, Nanocapsules/administration & dosage, Nanocapsules/chemistry, Neurons/chemistry, Peripheral Nerve Injuries/genetics, Peripheral Nerve Injuries/pathology, Peripheral Nerve Injuries/therapy, Plasmids/administration & dosage, Plasmids/genetics, Treatment Outcome, Biomedical engineering, Gene delivery, Axon, medicine.anatomical_structure, medicine, Denervation, Molecular biology, Neurotrophic factors, Pharmacology, Neuron, business.industry, business, Peripheral nerve injury
Related Organizations
Funded by
FCT| SFRH/BD/77933/2011
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: SFRH/BD/77933/2011
  • Funding stream: SFRH | Doutoramento
FCT| SFRH/BD/74304/2010
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: SFRH/BD/74304/2010
  • Funding stream: SFRH | Doutoramento
FCT| PTDC/CTM-NAN/115124/2009
TARGETS- Terapia génica direccionada para o tratamento de lesões nervosas
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: 115124
  • Funding stream: 5876-PPCDTI
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