publication . Article . Other literature type . 2017

BDNF gene delivery mediated by neuron-targeted nanoparticles is neuroprotective in peripheral nerve injury

Lopes, CDF; Gonçalves, NP; Gomes, CP; Saraiva, MJ; Pêgo, AP;
Open Access English
  • Published: 01 Mar 2017
  • Publisher: Elsevier
  • Country: Portugal
Abstract
Neuron-targeted gene delivery is a promising strategy to treat peripheral neuropathies. Here we propose the use of polymeric nanoparticles based on thiolated trimethyl chitosan (TMCSH) to mediate targeted gene delivery to peripheral neurons upon a peripheral and minimally invasive intramuscular administration. Nanoparticles were grafted with the non-toxic carboxylic fragment of the tetanus neurotoxin (HC) to allow neuron targeting and were explored to deliver a plasmid DNA encoding for the brain-derived neurotrophic factor (BDNF) in a peripheral nerve injury model. The TMCSH-HC/BDNF nanoparticle treatment promoted the release and significant expression of BDNF i...
Subjects
free text keywords: Brain-derived neurotrophic factor, Gene therapy, Neuron-targeted nanoparticles, Neuropathies, Neuroprotection, Trimethyl chitosan, Animals, Brain-Derived Neurotrophic Factor/genetics, Female, Genetic Therapy/methods, Mice, Mice Inbred BALB C, Nanocapsules/administration & dosage, Nanocapsules/chemistry, Neurons/chemistry, Peripheral Nerve Injuries/genetics, Peripheral Nerve Injuries/pathology, Peripheral Nerve Injuries/therapy, Plasmids/administration & dosage, Plasmids/genetics, Treatment Outcome, Biomedical engineering, Gene delivery, Axon, medicine.anatomical_structure, medicine, Denervation, Molecular biology, Neurotrophic factors, Pharmacology, Neuron, business.industry, business, Peripheral nerve injury
Related Organizations
Funded by
FCT| SFRH/BD/77933/2011
Project
SFRH/BD/77933/2011
CHITOSAN-BASED PLATFORM FOR TARGETED GENE DELIVERY TO PERIPHERAL NERVOUS SYSTEM
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: SFRH/BD/77933/2011
  • Funding stream: SFRH | Doutoramento
,
FCT| SFRH/BD/74304/2010
Project
SFRH/BD/74304/2010
THE PERIPHERAL NERVOUS SYSTEM IN TRANSTHYRETIN-RELATED AMYLOIDOSIS – MOLECULAR AND FUNCTIONAL STUDIES
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: SFRH/BD/74304/2010
  • Funding stream: SFRH | Doutoramento
,
FCT| PTDC/CTM-NAN/115124/2009
Project
PTDC/CTM-NAN/115124/2009
TARGETS- Terapia génica direccionada para o tratamento de lesões nervosas
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: 115124
  • Funding stream: 5876-PPCDTI
36 references, page 1 of 3

[5] J.F. Poduslo, G.L. Curran Permeability at the blood-brain and blood-nerve barriers of the neurotrophic factors: NGF, CNTF, NT-3, BDNF Brain Res. Mol. Brain Res., 36 (2) (1996), pp. 280-286 [OpenAIRE]

[6] J.F. Poduslo, G.L. Curran, J.S. Gill Putrescine-modified nerve growth factor: bioactivity, plasma pharmacokinetics, blood-brain/nerve barrier permeability, and nervous system biodistribution J. Neurochem., 71 (4) (1998), pp. 1651-1660

[7] H.U. Saragovi, K. Gehring Development of pharmacological agents for targeting neurotrophins and their receptors Trends Pharmacol. Sci., 21 (3) (2000), pp. 93-98 [OpenAIRE]

[8] J.C. Glorioso, M. Mata, D.J. Fink Therapeutic gene transfer to the nervous system using viral vectors J. Neurovirol., 9 (2) (2003), pp. 165-172

[9] A.P. Pêgo, H. Oliveira, P. Moreno Biomaterial-based vectors for targeted delivery of nucleic acids to the nervous system J. Coelho (Ed.), Drug Delivery Systems: Advanced Technologies Potentially Applicable in Personalised Treatment, Springer Netherlands (2013), pp. 185-224

[10] C.P. Gomes, C.D.F. Lopes, P.M.D. Moreno, A. Varela-Moreira, M.J. Alonso, A.P. Pêgo Translating chitosan to clinical delivery of nucleic acid-based drugs MRS Bull., 39 (01) (2014), pp. 60-70 [OpenAIRE]

[11] M. Thanou, B.I. Florea, M. Geldof, H.E. Junginger, G. Borchard Quaternized chitosan oligomers as novel gene delivery vectors in epithelial cell lines Biomaterials, 23 (1) (2002), pp. 153-159 [OpenAIRE]

[12] C.D. Lopes, C.P. Gomes, E. Neto, P. Sampaio, P. Aguiar, A.P. Pêgo Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles Nanomedicine (Lond.), 11 (24) (2016), pp. 3205- 3221 [OpenAIRE]

[13] C.D. Lopes, H. Oliveira, I. Estevao, L.R. Pires, A.P. Pêgo In vivo targeted gene delivery to peripheral neurons mediated by neurotropic poly(ethylene imine)-based nanoparticles Int. J. Nanomedicine, 11 (2016), pp. 2675-2683 [OpenAIRE]

[14] J.Y. Zhang, X.G. Luo, C.J. Xian, Z.H. Liu, X.F. Zhou Endogenous BDNF is required for myelination and regeneration of injured sciatic nerve in rodents Eur. J. Neurosci., 12 (12) (2000), pp. 4171-4180

[15] T. Fukuoka, E. Kondo, Y. Dai, N. Hashimoto, K. Noguchi Brain-derived neurotrophic factor increases in the uninjured dorsal root ganglion neurons in selective spinal nerve ligation model J. Neurosci., 21 (13) (2001), pp. 4891-4900 [OpenAIRE]

[16] H. Kobayashi, M. Yokoyama, Y. Matsuoka, M. Omori, Y. Itano, R. Kaku, K. Morita, H. Ichikawa Expression changes of multiple brain-derived neurotrophic factor transcripts in selective spinal nerve ligation model and complete Freund's adjuvant model Brain Res., 1206 (2008), pp. 13-19

[23] C. Wan, N.N. Liu, L.M. Liu, N. Cai, L. Chen Effect of adenovirus-mediated brain derived neurotrophic factor in early retinal neuropathy of diabetes in rats Int. J. Ophthalmol., 3 (2) (2010), pp. 145-148

[24] H. Fukui, H.T. Wong, L.A. Beyer, B.G. Case, D.L. Swiderski, A. Di Polo, A.F. Ryan, Y. Raphael BDNF gene therapy induces auditory nerve survival and fiber sprouting in deaf Pou4f3 mutant mice Sci. Rep., 2 (2012), p. 838

[25] T. Nakaizumi, K. Kawamoto, R. Minoda, Y. Raphael Adenovirus-mediated expression of brain-derived neurotrophic factor protects spiral ganglion neurons from ototoxic damage Audiol. Neurotol., 9 (3) (2004), pp. 135-143

36 references, page 1 of 3
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