publication . Article . Preprint . 2016

Robust gap repair in the contractile ring ensures timely completion of cytokinesis.

Inês Mendes Pinto; Ana Marta Silva; Ana Marta Silva; Ivo A. Telley; Ana Carvalho; Ana Carvalho; Boris Rubinstein; Reto Gassmann; Reto Gassmann; Daniel S. Osório; ...
Open Access English
  • Published: 21 May 2016 Journal: The Journal of Cell Biology, volume 215, issue 6, pages 789-799 (issn: 0021-9525, eissn: 1540-8140, Copyright policy)
  • Publisher: The Rockefeller University Press
  • Country: Portugal
Abstract
Cytokinesis in animal cells requires the constriction of an actomyosin contractile ring, whose architecture and mechanism remain poorly understood. We use laser microsurgery to explore the biophysical properties of constricting rings in Caenorhabditis elegans embryos. Laser cutting causes rings to snap open. However, instead of disintegrating, ring topology recovers and constriction proceeds. In response to severing, a finite gap forms and is repaired by recruitment of new material in an actin polymerization–dependent manner. An open ring is able to constrict, and rings repair from successive cuts. After gap repair, an increase in constriction velocity allows cy...
Subjects
Medical Subject Headings: cardiovascular systemcirculatory and respiratory physiology
free text keywords: Research Articles, Report, 38, 21, 10, Cell Biology, Actomyosin/metabolism, Animals, Bridged Bicyclo Compounds, Heterocyclic/pharmacology, Caenorhabditis elegans/cytology, Caenorhabditis elegans/drug effects, Caenorhabditis elegans/embryology, Embryo, Nonmammalian/cytology, Lasers, Microsurgery, Thiazolidines/pharmacology, Caenorhabditis elegans embryos, ring constriction, Actomyosin contractile ring, Ring network, Cytokinesis, Biophysics, Laser microsurgery, Ingression, Biology, Actin, Anatomy, Laser cutting, Constriction
Funded by
EC| PRECISE
Project
PRECISE
Spatiotemporal regulation of chromosome segregation fidelity
  • Funder: European Commission (EC)
  • Project Code: 260892
  • Funding stream: FP7 | SP2 | ERC
,
FCT| PTDC/BEX-BCM/0654/2012
Project
PTDC/BEX-BCM/0654/2012
Actin and myosin II in the contractile ring - mechanisms of constriction
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: 125211
  • Funding stream: COMPETE
,
EC| ACTOMYO
Project
ACTOMYO
Mechanisms of actomyosin-based contractility during cytokinesis
  • Funder: European Commission (EC)
  • Project Code: 640553
  • Funding stream: H2020 | ERC | ERC-STG
,
EC| DYNEINOME
Project
DYNEINOME
Cytoplasmic Dynein: Mechanisms of Regulation and Novel Interactors
  • Funder: European Commission (EC)
  • Project Code: 338410
  • Funding stream: FP7 | SP2 | ERC
,
FCT| SFRH/BPD/95707/2013
Project
SFRH/BPD/95707/2013
Contractile Ring Dynamics and Mechanism of Constriction During Cytokinesis
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: SFRH/BPD/95707/2013
  • Funding stream: SFRH | Pós-Doutoramento

Carvalho, A., A. Desai, and K. Oegema. 2009. Structural memory in the contractile ring makes the duration of cytokinesis independent of cell size. Cell. 137:926-937. http://dx.doi.org/10.1016/j.cell.2009.03.021

Related research
Abstract
Cytokinesis in animal cells requires the constriction of an actomyosin contractile ring, whose architecture and mechanism remain poorly understood. We use laser microsurgery to explore the biophysical properties of constricting rings in Caenorhabditis elegans embryos. Laser cutting causes rings to snap open. However, instead of disintegrating, ring topology recovers and constriction proceeds. In response to severing, a finite gap forms and is repaired by recruitment of new material in an actin polymerization–dependent manner. An open ring is able to constrict, and rings repair from successive cuts. After gap repair, an increase in constriction velocity allows cy...
Subjects
Medical Subject Headings: cardiovascular systemcirculatory and respiratory physiology
free text keywords: Research Articles, Report, 38, 21, 10, Cell Biology, Actomyosin/metabolism, Animals, Bridged Bicyclo Compounds, Heterocyclic/pharmacology, Caenorhabditis elegans/cytology, Caenorhabditis elegans/drug effects, Caenorhabditis elegans/embryology, Embryo, Nonmammalian/cytology, Lasers, Microsurgery, Thiazolidines/pharmacology, Caenorhabditis elegans embryos, ring constriction, Actomyosin contractile ring, Ring network, Cytokinesis, Biophysics, Laser microsurgery, Ingression, Biology, Actin, Anatomy, Laser cutting, Constriction
Funded by
EC| PRECISE
Project
PRECISE
Spatiotemporal regulation of chromosome segregation fidelity
  • Funder: European Commission (EC)
  • Project Code: 260892
  • Funding stream: FP7 | SP2 | ERC
,
FCT| PTDC/BEX-BCM/0654/2012
Project
PTDC/BEX-BCM/0654/2012
Actin and myosin II in the contractile ring - mechanisms of constriction
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: 125211
  • Funding stream: COMPETE
,
EC| ACTOMYO
Project
ACTOMYO
Mechanisms of actomyosin-based contractility during cytokinesis
  • Funder: European Commission (EC)
  • Project Code: 640553
  • Funding stream: H2020 | ERC | ERC-STG
,
EC| DYNEINOME
Project
DYNEINOME
Cytoplasmic Dynein: Mechanisms of Regulation and Novel Interactors
  • Funder: European Commission (EC)
  • Project Code: 338410
  • Funding stream: FP7 | SP2 | ERC
,
FCT| SFRH/BPD/95707/2013
Project
SFRH/BPD/95707/2013
Contractile Ring Dynamics and Mechanism of Constriction During Cytokinesis
  • Funder: Fundação para a Ciência e a Tecnologia, I.P. (FCT)
  • Project Code: SFRH/BPD/95707/2013
  • Funding stream: SFRH | Pós-Doutoramento

Carvalho, A., A. Desai, and K. Oegema. 2009. Structural memory in the contractile ring makes the duration of cytokinesis independent of cell size. Cell. 137:926-937. http://dx.doi.org/10.1016/j.cell.2009.03.021

Related research
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