publication . Article . 2007

Characterization of pancreatic transcription factor Pdx-1 binding sites using promoter microarray and serial analysis of chromatin occupancy

David M. Keller; Shannon K. McWeeney; Athanasios Arsenlis; Jacques Drouin; Christopher V.E. Wright; Haiyan Wang; Claes B. Wollheim; Peter White; Klaus H. Kaestner; Richard H. Goodman;
Open Access English
  • Published: 01 Jan 2007
  • Country: Switzerland
The homeobox transcription factor Pdx-1 is necessary for pancreas organogenesis and beta cell function, however, most Pdx-1-regulated genes are unknown. To further the understanding of Pdx-1 in beta cell biology, we have characterized its genomic targets in NIT-1 cells, a mouse insulinoma cell line. To identify novel targets, we developed a microarray that includes traditional promoters as well as non-coding conserved elements, micro-RNAs, and elements identified through an unbiased approach termed serial analysis of chromatin occupancy. In total, 583 new Pdx-1 target genes were identified, many of which contribute to energy sensing and insulin release in pancre...
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Medical Subject Headings: endocrine systemhormones, hormone substitutes, and hormone antagonists
free text keywords: Animals, Basic Helix-Loop-Helix Transcription Factors/metabolism, Binding Sites, Cell Differentiation, Chromatin/metabolism, Chromatin Immunoprecipitation, Homeodomain Proteins/metabolism, Insulin, Insulin-Secreting Cells/cytology/metabolism, Mice, Mice, Inbred C57BL, MicroRNAs/metabolism, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Promoter Regions, Genetic, Trans-Activators/metabolism, Transcription, Genetic, Cell Biology, Biochemistry, Molecular Biology, ddc:612, Biology, Transcription factor, Pancreas, medicine.anatomical_structure, medicine, Homeobox, Chromatin, Gene, Beta cell, Promoter, NEUROD1
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