publication . Article . 2004

Overexpression of TIMP-1 under the MMP-9 promoter interferes with wound healing in transgenic mice

Tuire Salonurmi; Mataleena Parikka; Sirpa Kontusaari; Emma Piril�; Carine Munaut; Tuula Salo; Karl Tryggvason;
Open Access English
  • Published: 01 Jan 2004
  • Country: Belgium
Abstract
We have generated transgenic mice harboring the murine matrix metalloproteinase 9 (MMP-9) promoter cloned in front of human TIMP-1 cDNA. The transgenic mice were viable and fertile and exhibited normal growth and general development. During wound healing the mice were shown to express human TIMP-1 in keratinocytes that normally express MMP-9. However, the healing of skin wounds was significantly retarded with slow migration of keratinocytes over the wound in transgenic mice. In situ zymography carried out on wound tissues revealed total blockage of gelatinolytic activity (i.e., MMP-9 and MMP-2). The results confirm studies with MMP-9 knockout mice showing that M...
Subjects
free text keywords: gene expression regulation, keratinocytes, matrix metalloproteinases, tissue inhibitor of metalloproteinase-1, transgenic mice, : Biochemistry, biophysics & molecular biology [Life sciences], : Biochimie, biophysique & biologie moléculaire [Sciences du vivant], Pathology and Forensic Medicine, Cell Biology, Histology, Matrix metalloproteinase, Biology, Wound healing, Keratinocyte, medicine.anatomical_structure, medicine, Anatomy, Knockout mouse, Zymography, Genetically modified mouse, Molecular medicine, Complementary DNA
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publication . Article . 2004

Overexpression of TIMP-1 under the MMP-9 promoter interferes with wound healing in transgenic mice

Tuire Salonurmi; Mataleena Parikka; Sirpa Kontusaari; Emma Piril�; Carine Munaut; Tuula Salo; Karl Tryggvason;