publication . Other literature type . Article . 1999

Housekeeping genes as internal standards: use and limits.

Thierry Grisar; Ahmed Igout; Bernard Lakaye; B. de Borman; Georges Hennen; Olivier Thellin; Ernst Heinen; Willy Zorzi; Bernard Coumans;
Open Access
  • Published: 01 Oct 1999
  • Publisher: Elsevier BV
  • Country: Belgium
Abstract
Quantitative studies are commonly realised in the biomedical research to compare RNA expression in different experimental or clinical conditions. These quantifications are performed through their comparison to the expression of the housekeeping gene transcripts like glyceraldehyde-3-phosphate dehydrogenase (G3PDH), albumin, actins, tubulins, cyclophilin, hypoxantine phsophoribosyltransferase (HRPT), L32. 28S, and 18S rRNAs are also used as internal standards. In this paper, it is recalled that the commonly used internal standards can quantitatively vary in response to various factors. Possible variations are illustrated using three experimental examples. Preferr...
Subjects
Medical Subject Headings: education
free text keywords: Biotechnology, Applied Microbiology and Biotechnology, General Medicine, Amplification, RNA, PCR, : Biochemistry, biophysics & molecular biology [Life sciences], : Biochimie, biophysique & biologie moléculaire [Sciences du vivant], Rna expression, Housekeeping gene, Rnase protection, Genetics, Biology, Reference genes
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