publication . Article . 2005

Oxidative stress and DNA damage caused by the urban air pollutant 3-NBA and its isomer 2-NBA in human lung cells analyzed with three independent methods

Nagy, Eszter; Johansson, Clara; Zeisig, Magnus; Moller, Lennart;
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  • Published: 15 Nov 2005 Journal: Journal of Chromatography B, volume 827, pages 94-103 (issn: 1570-0232, Copyright policy)
  • Publisher: Elsevier BV
  • Country: Poland
Abstract
Abstract The air pollutant 3-nitrobenzanthrone (3-NBA), emitted in diesel exhaust, is a potent mutagen and genotoxin. 3-NBA can isomerise to 2-nitrobenzanthrone (2-NBA), which can become more than 70-fold higher in concentration in ambient air. In this study, three independent methods have been employed to evaluate the oxidative stress and genotoxicity of 2-NBA compared to 3-NBA in the human A549 lung cell line. HPLC–EC/UV was applied for measurements of oxidative damage in the form of 8-oxo-2′-deoxyguanosine (8-oxodG), 32P-HPLC for measurements of lipophilic DNA-adducts, and the Comet assay to measure a variety of DNA lesions, including oxidative stress. No sig...
Subjects
free text keywords: Air pollutant, Benz(a)anthracenes, Toxicity, Humans, Lung, Chromatography, High Pressure Liquid, Analytical Chemistry, Clinical Biochemistry, Cell Biology, Biochemistry, General Medicine, DNA damage, Comet assay, Oxidative stress, medicine.disease_cause, medicine, Genotoxicity, Chromatography, Pollutant, 3-Nitrobenzanthrone, chemistry.chemical_compound, chemistry, Mutagen, Diesel exhaust
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publication . Article . 2005

Oxidative stress and DNA damage caused by the urban air pollutant 3-NBA and its isomer 2-NBA in human lung cells analyzed with three independent methods

Nagy, Eszter; Johansson, Clara; Zeisig, Magnus; Moller, Lennart;