publication . Thesis . 2006

Molecular characterization and expression profiling of cd-responsive genes in triticum durum

Cebeci, Özge; Cebeci, Ozge;
Open Access
  • Published: 01 Jan 2006
  • Country: Yemen
Abstract
Cadmium (Cd) is a toxic heavy metal which has detrimental effects both in plants and human. There is a lack of knowledge on the molecular mechanisms of Cd toxicity in crop plants. The objective of this study was to identify and clone expressed Cd-responsive genes from two Triticum durum cvs. Balcah-85 and Balcah-2000 using mRNA differential display technique. We identified 10 cDNA clones whose level of expression significantly changed upon Cd exposure and thus isolated for further characterization. Differential expression patterns of cDNA clones were verified by Northern blot and Real-Time PCR analyses. Analysis of putative protein sequences of these clones reve...
Subjects
free text keywords: TA Engineering (General). Civil engineering (General)
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