publication . Article . 1994

Differentiation of human lymphocytes into nuclear vlimata by meiosis. The cytotoxic effect of calcium-activated neutral proteinase inhibitor

Logothetou-Rella, H.;
Open Access English
  • Published: 01 Jan 1994
  • Publisher: Murcia : F. Hernández
  • Country: Spain
Phytohaemagglutinin (PHA)-activated lymphocytes differentiated into nuclear vlimata (NVs) in vitro. Lymphocyte attachment was followed by formation and extrusion of cytoplasmic vesicles. nuclear elongation and fragmentation into NVs. NVs and cytoplasmic vesicles were detached and organized into large cell nodules in suspension. Immunocytochemistry showed that T-lymphocytes differentiated mainly to NVs while B-lymphocytes to buds. During differentiation there was a loss or gain of T-antigenicity by either mother or daughter cell. Cytogenetic analysis by chromosomal spreading and in situ techniques showed that NVs do carry chromosomes of hypodiploid or hypohaploid...
free text keywords: Lymphocyte, Cytogenetics, :6 - Ciencias aplicadas::61 - Medicina [CDU]
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