publication . Article . Other literature type . 2008

Potential role of NADPH-oxidase in early steps of lead-induced oxidative burst in Vicia faba roots

Pourrut, B.; Perchet, G.; Silvestre, J.; Cecchi, M.; Guiresse, M.; Pinelli, E.;
Open Access English
  • Published: 18 Apr 2008 Journal: Journal of Plant Physiology (issn: 0176-1617, Copyright policy)
  • Publisher: Elsevier
  • Country: France
Abstract
The mechanism of oxidative burst induced by lead in Vicia faba excised roots was investigated by luminol-dependent chemiluminescence. Results showed that lead triggered a rapid and dose-dependent increase in chemiluminescence production. In this study, specific inhibitors of putative reactive oxygen species (ROS) sources were used to determine the mechanism of lead-induced ROS generation. This generation was sensitive to dephenylene iodonium (DPI), quinacrine and imidazole, some inhibitors of the NADPH-oxidase and not inhibited by other putative ROS sources inhibitors. Data reported in this work clearly demonstrated the pivotal role of NADPH-oxidase-like enzyme i...
Subjects
free text keywords: Biodiversité et Ecologie, Ecologie, Environnement, Lead, NADPH-oxidase, Oxidative burst, Reactive oxygen species, Vicia faba, Agronomy and Crop Science, Plant Science, Physiology, Respiratory burst, NADPH oxidase, biology.protein, biology, chemistry.chemical_classification, chemistry, Calcium, chemistry.chemical_element, Calmodulin, Protein kinase C, Botany, Staurosporine, medicine.drug, medicine, Biochemistry
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publication . Article . Other literature type . 2008

Potential role of NADPH-oxidase in early steps of lead-induced oxidative burst in Vicia faba roots

Pourrut, B.; Perchet, G.; Silvestre, J.; Cecchi, M.; Guiresse, M.; Pinelli, E.;