publication . Article . 2009

A new cell primo-culture method for freshwater benthic diatom communities

Debenest, T.; Silvestre, J.; Coste, Marine; Delmas, F.; Pinelli, Eric;
Open Access English
  • Published: 01 Jan 2009
  • Publisher: HAL CCSD
  • Country: France
Abstract
A new cell primo-culture method was developed for the benthic diatom community isolated from biofilm sampled in rivers. The approach comprised three steps: (1) scraping biofilm from river pebbles, (2) diatom isolation from biofilm, and (3) diatom community culture. With a view to designing a method able to stimulate the growth of diatoms, to limit the development of other microorganisms, and to maintain in culture a community similar to the original natural one, different factors were tested in step 3: cell culture medium (Chu No 10 vs Freshwater “WC” medium modified), cell culture vessel, and time of culture. The results showed that using Chu No 10 medium in an...
Subjects
Medical Subject Headings: fungi
free text keywords: Biodiversité et Ecologie, Ecologie, Environnement, Interactions entre organismes, Diatom community, Culture, Biofilm, Plant Science, Aquatic Science, [SDE]Environmental Sciences
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