publication . Conference object . 2011

Polymeric gel nanoparticle pH sensors for intracellular measurements

Thomas Lars Andresen;
  • Published: 01 Jan 2011
  • Country: Denmark
Abstract
Precise measurements of pH in cells and intracellular compartments are of importance to both the fundamental understanding of metabolism and to the development of drugs that are released from the endosomes-lysome pathway. We have developed polymer gel nanoparticles as carriers of covalently bound fluorophores for ratiometric measurements of pH. One pH insensitive fluorophore serves as a reference while one or more pH sensitive fluorophores serve to give the desired pH dependence of the output. With one pH sensitive fluorophore a dynamic range of ~2 pH units is achieved. The physiologically interesting pH range is approximately 4 pH units and thus a nanoparticle ...
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publication . Conference object . 2011

Polymeric gel nanoparticle pH sensors for intracellular measurements

Thomas Lars Andresen;