publication . Doctoral thesis . 2000

Modern methods in cereal grain mycology

Olsson, Johan;
Open Access
  • Published: 01 Jan 2000
  • Country: Sweden
Abstract
A simple rapid DNA extraction method, equally suitable for spores and mycelia is proposed. Heating samples in NaOH and SDS provides DNA of high purity, suitable for Polymerase Chain Reaction (PCR) analysis. For Penicillium roqueforti the detection limit was 6 x lo3 conidia and 1 mg (fresh weight) mycelium in the extraction liquid. The method proved efficient with Aspergillus jlavus, Fusarium graminearum, Rhizopus stolonifer, Eurotium herbariorum, and Cladosporium herbarum, as well. An optimised competitive PCR (cPCR) method for quantifying fungal growth in cereal grain was developed using experimental design. DNA extraction efficiency was quantified by cPCR usin...
Subjects
free text keywords: Agris categories are used [ZZZ placeholder]
Download from
Epsilon Open Archive
Doctoral thesis . 2000
123 references, page 1 of 9

Akerstrand, K. (1995). Mould and yeast. Determination in foods. UDC 582.23:633.1. Nordic Committee on Food Analysis.

Albano, C., Blomqvist, G., Coomans, D., Dunn 111, W. J., Edlund, U., Eliasson, B., Hellberg, S., Johansson, E., Norden, B., Sjostrom, M., Soderstrom, B., Wold, H. & Wold, S. (1981). Pattern recognition by means of disjoint principal components models (SIMCA). Philosophy and methods. In Proceedings Symposium i Anvent Statistik, pp. 183-218. Edited by A. E. A. Hoskuldsson. Copenhagen: NEUCC, RECAU and RECKU.

Arnold, B. L., Itakura, K. & Rossi, B. T. (1992). PCR-based quantitation of low levels of HIV-1 DNA by using an external standard. Genet. Anal. Tech. Appl. 9, 113-116.

Bachinger, T., Martensson, P. & Mandenius, C. F. (1998). Estimation of biomass and specific growth rate in a recombinant Escherichia coli batch cultivation process using a chemical multisensor array. J. Biotech. 60,55-66.

Bachinger, T., Riese, U., Eriksson, R. & Mandenius, C. F. (2000). Monitoring cellular state transitions in a production-scale CHO-cell process using an electronic nose. J. Biotech. 76, 61-71. [OpenAIRE]

Bartnicki-Garcia, S. & Lippman, E. (1982). Fungal cell wall composition. In CRS Handbook of Microbiology, pp. 229-252. Edited by A. I. Laskin & H. A. Lechevalier. Vleveland, Ohio: Boca Raton

Bennett, J. W. (1995). From molecular genetics and secondary metabolism to molecular metabolites and secondary genetics. Can. J. But. 73, S917-S924.

Boysen, M. E., Eriksson, A. R. B. & Schniirer, J. (2000). Detection of food-borne toxigenic molds using molecular prtbes. in press.

Breitholtz, A., Olsen, M., Dahlback, A. & Hult, K. (1991). Plasma ochratoxin A levels in three Swedish populations surveyed using an ion-pair HPLC technique. Food Addit. Contam. 8, 183-192.

Brezmes, J., Ferreras, B., Llobet, E., Vilanova, X. & Correig, X. (1997). Neural network based electronic nose for the classification of aromatic species. Anal. Chim. Acta 348, 503-509. [OpenAIRE]

Borjesson, T. (1993). Volatilefungal metabolites as indicators of mould growth in stored cereals, Dept. of Microbiology 55, Swedish University of Agricultural Sciences, Uppsala.

Borjesson, T., Eklov, T., Jonsson, A., Sundgren, H. & Schnurer, J. (1996). Electronic nose for odor classification of grains. Cereal Chem. 73,457-461.

Cabib, E., Roberts, R. & Bowere, B. (1982). Synthesis of the yeast cell wall and its regulation. Ann. Rev. Microbiol. 5 1,763-793.

Campbell, I. M. (1984). Secondary metabolism and microbial physiology. In Advances in Microbial Physiology, pp. 1-60. Edited by A. H. Rose & D. W. Tempest. London: Academic Press.

Chelkowski, J. (1991). Cereal grain - Mycotoxins, fungi and quality in drying and storage. In Developments in Food Sciences, pp. xiii-xx. Edited by Chelkowski, J. Amsterdam: Elsevier Science.

123 references, page 1 of 9
Powered by OpenAIRE Open Research Graph
Any information missing or wrong?Report an Issue