publication . Doctoral thesis . 2000

Modern methods in cereal grain mycology

Olsson, Johan;
Open Access
  • Published: 01 Jan 2000
  • Country: Sweden
A simple rapid DNA extraction method, equally suitable for spores and mycelia is proposed. Heating samples in NaOH and SDS provides DNA of high purity, suitable for Polymerase Chain Reaction (PCR) analysis. For Penicillium roqueforti the detection limit was 6 x lo3 conidia and 1 mg (fresh weight) mycelium in the extraction liquid. The method proved efficient with Aspergillus jlavus, Fusarium graminearum, Rhizopus stolonifer, Eurotium herbariorum, and Cladosporium herbarum, as well. An optimised competitive PCR (cPCR) method for quantifying fungal growth in cereal grain was developed using experimental design. DNA extraction efficiency was quantified by cPCR usin...
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Doctoral thesis . 2000
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