Mapping of yield, yield stability, yield adaptability and other traits in barley using linkage disequilibrium mapping and linkage analysis

Doctoral thesis English OPEN
Kraakman, A.T.W. (2005)
  • Publisher: s.n.

Plants is mostly done through linkage analysis. A segregating mapping population Identification and mappping of Quantitative Trait Loci (QTLs) in is created from a bi-parental cross and linkages between trait values and mapped markers reveal the positions of<span class=SpellE>QTLs</span>. In<span class=SpellE>this</span><span class=SpellE>study</span><span class=SpellE>we</span><span class=SpellE>explored</span>linkage<span class=SpellE>disequilibrium</span>(LD)<span class=SpellE>mapping</span>of traits in a set of modern<span class=SpellE>barley</span>cultivars. LD<span class=SpellE>between</span><span class=SpellE>molecular</span>markers<span class=SpellE>was</span><span class=SpellE>found</span>up to a distance of 10 centimorgan,<span class=SpellE>which</span><span class=SpellE>is</span>large<span class=SpellE>compared</span>to<span class=SpellE>other</span><span class=SpellE>species</span>.<span class=SpellE>The</span>large distance<span class=SpellE>might</span><span class=SpellE>be</span><span class=SpellE>induced</span>by LD<span class=SpellE>increasing</span><span class=SpellE>factors</span><span class=SpellE>such</span>as<span class=SpellE>inbreeding</span><span class=SpellE>and</span><span class=SpellE>the</span><span class=SpellE>fact</span><span class=SpellE>that</span><span class=SpellE>the</span>population<span class=SpellE>is</span><span class=SpellE>most</span><span class=SpellE>likely</span><span class=SpellE>based</span>on a<span class=SpellE>rather</span><span class=SpellE>small</span>set of<span class=SpellE>founding</span><span class=SpellE>genotypes</span>. Associations<span class=SpellE>between</span>markers<span class=SpellE>and</span>traits<span class=SpellE>were</span><span class=SpellE>found</span>for<span class=SpellE>yield</span>,<span class=SpellE>yield</span><span class=SpellE>stability</span>,<span class=SpellE>leaf</span><span class=SpellE>rust</span><span class=SpellE>resistance</span>(LR),<span class=SpellE>barley</span><span class=SpellE>yellow</span><span class=SpellE>dwarf</span>virus<span class=SpellE>resistance</span>(BYD), plant<span class=SpellE>height</span>,<span class=SpellE>and</span><span class=SpellE>days</span>to<span class=SpellE>heading</span>.<span class=SpellE>Trait-associated</span>markers<span class=SpellE>from</span>LD<span class=SpellE>analysis</span><span class=SpellE>were</span><span class=SpellE>located</span>in<span class=SpellE>regions</span><span class=SpellE>where</span><span class=SpellE>already</span><span class=SpellE>QTLs</span>for<span class=SpellE>the</span>trait<span class=SpellE>considered</span><span class=SpellE>had</span>been<span class=SpellE>reported</span><span class=SpellE>from</span><span class=SpellE>studies</span><span class=SpellE>based</span>on<span class=SpellE>bi-parental</span>crosses. In addition, new<span class=SpellE>QTLs</span><span class=SpellE>were</span><span class=SpellE>found</span>for<span class=SpellE>yield</span>,<span class=SpellE>yield</span><span class=SpellE>stability</span>, LR<span class=SpellE>and</span>BYD.<span class=SpellE>We</span><span class=SpellE>expect</span><span class=SpellE>that</span>LD<span class=SpellE>mapping</span><span class=SpellE>will</span><span class=SpellE>become</span>a<span class=SpellE>valuable</span>extension to<span class=SpellE>conventional</span>QTL<span class=SpellE>analysis</span>in plant<span class=SpellE>breeding</span>.<o:p></o:p></span>Special</span></span><span lang=FR style='mso-ansi-language:FR'>attention<span class=SpellE>was</span><span class=SpellE>given</span>to traits<span class=SpellE>describing</span><span class=SpellE>genotype</span>×<span class=SpellE>environment</span>interactions.<span class=SpellE>Statistical</span>models<span class=SpellE>were</span><span class=SpellE>used</span>to<span class=SpellE>define</span><span class=SpellE>measures</span>for<span class=SpellE>yield</span><span class=SpellE>adaptability</span><span class=SpellE>and</span><span class=SpellE>yield</span><span class=SpellE>stability</span><span class=SpellE><span class=GramE>without</span></span><span class=SpellE>including</span><span class=SpellE>environmental</span><span class=SpellE>factors</span><span class=SpellE>directly</span>in<span class=SpellE>the</span>models.<span class=SpellE>Adaptability</span><span class=SpellE>was</span><span class=SpellE>defined</span>as<span class=SpellE>the</span><span class=SpellE>responsiveness</span>of<span class=SpellE>the</span><span class=SpellE>genotype</span>to<span class=SpellE>the</span><span class=SpellE>environment</span>,<span class=SpellE>and</span><span class=SpellE>stability</span><span class=SpellE>was</span><span class=SpellE>defined</span>as<span class=SpellE>the</span><span class=SpellE>unexplained</span><span class=SpellE>deviation</span><span class=SpellE>from</span><span class=SpellE>the</span><span class=SpellE>statistical</span>model. LD<span class=SpellE>mapping</span>in<span class=SpellE>barley</span>cultivars<span class=SpellE>resulted</span>in<span class=SpellE>marker-trait</span>associations for<span class=SpellE>yield</span><span class=SpellE>stability</span>. In addition, linkage analyses in four<span class=SpellE>doubled-haploid</span>populations<span class=SpellE>resulted</span>in<span class=SpellE>detection</span>of<span class=SpellE>many</span><span class=SpellE>QTLs</span>for<span class=SpellE>adaptability</span>, but<span class=SpellE>only</span>a single QTL for<span class=SpellE>stability</span>.<span class=SpellE>We</span><span class=SpellE>concluded</span><span class=SpellE>that</span><span class=SpellE>adaptability</span><span class=SpellE>measures</span><span class=SpellE>were</span><span class=SpellE>genetically</span><span class=SpellE>better</span><span class=SpellE>defined</span><span class=SpellE>than</span><span class=SpellE>stability</span><span class=SpellE>measures</span><span class=SpellE>and</span><span class=SpellE>that</span><span class=SpellE>selection</span>for<span class=SpellE>adaptability</span><span class=SpellE>should</span><span class=SpellE>be</span>possible.
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