publication . Article . 2014

Tracing carbon flow from microphytobenthos to major bacterial groups in an intertidal marine sediment by using an in situ 13C pulse-chase method

Miyatake, T.; Moerdijk-Poortvliet, T.C.W.; Stal, L.J.; Boschker, H.T.S.;
Open Access English
  • Published: 01 Jan 2014
  • Country: Netherlands
Abstract
Carbon flow from benthic diatoms to heterotrophic bacterial was traced in an intertidal sediment for 5 consecutive days. 13C-labeled bicarbonate was sprayed onto the sediment surface during low tide and 13C-label incorporation in major carbon pools, intermediate metabolites, and biomarkers were monitored. Phospholipid-derived fatty acid (PLFA) and ribosomal ribonucleic acid (rRNA) were used to identify the responsible members of the microbial community at class and family phylogenetic resolution. Diatoms were the predominant primary producers, and Gammaproteobacteria, Bacteroidetes, and Deltaproteobacteria (21%, 8%, and 7% of 16S rRNA-derived clone library) were...
Subjects
Medical Subject Headings: fungi
Related Organizations
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