publication . Conference object . 2013

Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors

De, Arpita; van Nieuwkasteele, Jan William; Carlen, Edwin; van den Berg, Albert;
  • Published: 16 Jun 2013
  • Publisher: IEEE
  • Country: Netherlands
Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA≈7×106 M-1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be ~1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a...
free text keywords: EWI-24119, METIS-300227, IR-88285
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Conference object . 2013
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