publication . Article . 1984

Lateral mobility of plasma membrane lipids in dividing Xenopus eggs.

P.A.T. Tetteroo; J.G. Bluemink; W.J.A.G. Dictus; E.J.J. van Zoelen; S.W. de Laat;
Open Access English
  • Published: 01 Jan 1984
  • Country: Netherlands
Abstract The lateral mobility of plasma membrane lipids was analyzed during first cleavage of Xenopus laevis eggs by fluorescence photobleaching recovery (FPR) measurements, using the lipid analogs 5-(N-hexadecanoyl)aminofluorescein (“HEDAF”) and 5-(N-tetradecanoyl)aminofluorescein (“TEDAF”) as probes. The preexisting plasma membrane of the animal side showed an inhomogeneous, dotted fluorescence pattern after labeling and the lateral mobility of both probes used was below the detection limits of the FPR method (D ⪡ 10−10cm2/sec). In contrast, the preexisting plasma membrane of the vegetal side exhibited homogeneous fluorescence and the lateral diffusion coeffic...
free text keywords: Membrane fluidity, Biology, Fluorescence, Cell membrane, medicine.anatomical_structure, medicine, Cleavage (embryo), Membrane lipids, Anatomy, Membrane, Xenopus, biology.organism_classification, Fluorescein, chemistry.chemical_compound, chemistry
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