publication . Article . 1984

Lateral mobility of plasma membrane lipids in dividing Xenopus eggs.

Laat, S.W. de; Tetteroo, P.A.T.; Bluemink, J.G.; Dictus, W.J.A.G.; Zoelen, E.J.J. van;
Open Access
  • Published: 01 Jan 1984 Journal: Developmental Biology, volume 104, pages 210-218 (issn: 0012-1606, Copyright policy)
  • Publisher: Elsevier BV
  • Country: Netherlands
Abstract
Abstract The lateral mobility of plasma membrane lipids was analyzed during first cleavage of Xenopus laevis eggs by fluorescence photobleaching recovery (FPR) measurements, using the lipid analogs 5-( N -hexadecanoyl)aminofluorescein (“HEDAF”) and 5-( N -tetradecanoyl)aminofluorescein (“TEDAF”) as probes. The preexisting plasma membrane of the animal side showed an inhomogeneous, dotted fluorescence pattern after labeling and the lateral mobility of both probes used was below the detection limits of the FPR method ( D ⪡ 10 −10 cm 2 /sec). In contrast, the preexisting plasma membrane of the vegetal side exhibited homogeneous fluorescence and the lateral diffusio...
Related Organizations
Powered by OpenAIRE Research Graph
Any information missing or wrong?Report an Issue