publication . Article . 1979

Bacteriophage φX174 RF DNA replication in vivo: A study by electron microscopy☆

W. Keegstra; P.D. Baas; H.S. Jansz;
Open Access English
  • Published: 25 Nov 1979
  • Country: Netherlands
Abstract
We have investigated bacteriophage φX174 RF † DNA replication by electron microscopy. Three different, types of replicative intermediates were observed: rolling circles, partially duplex DNA circles and structures consisting of two DNA circles connected at a single point. Rolling circles with a single-stranded or partially double-stranded DNA tail were both observed. After cleavage of the rolling circles with the restriction endonuclease from Providentia stuartii 164 (PstI) the startpoint of rolling circle replication could be located at 21 map units from the PstI cleavage site in agreement with the previously determined position of the origin of φX RF DNA repli...
Subjects
free text keywords: Molecular Biology, Rolling circle replication, DNA, chemistry.chemical_compound, chemistry, DNA replication, Circular bacterial chromosome, Cleavage (embryo), Complementary DNA, DNA polymerase, biology.protein, biology, Restriction enzyme
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publication . Article . 1979

Bacteriophage φX174 RF DNA replication in vivo: A study by electron microscopy☆

W. Keegstra; P.D. Baas; H.S. Jansz;