publication . Article . 1994

A strategy for the mapping of N-glycans by high-performance capillary electrophoresis

Vliegenthart, J.F.G.; Hermentin, P.; Doenges, R.; Witzel, R.; Hokke, C.H.; Kamerling, J.P.; Conradt, H.S.; Nimtz, M.;
Open Access English
  • Published: 01 Jan 1994
  • Country: Netherlands
Abstract
We have evaluated high-performance capillary electrophoresis (HPCE) with respect to its suitability for use in establishing a carbohydrate-mapping database that would enable a carbohydrate structural analysis by mere comparison of migration times. The suitability of HPCE for carbohydrate structural assignments was ascertained by validation experiments. The migration times of distinct N-glycans, prepared and measured on different days, were shown to be highly reproducible, with a coefficient of variation of usually less than 0.20%, requiring only femtomoles of N-glycan per injection for reliable measurements. By including mesityl oxide and sialic acid as internal...
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