publication . Article . 2003

Efficient screening methods for glucosyltransferase genes in Lactobacillus strains

Kralj, S; van Geel-schutten, GH; van der Maarel, MJEC; Dijkhuizen, L;
Closed Access English
  • Published: 01 Aug 2003
  • Country: Netherlands
Abstract
Limited information is available about homopolysaccharide synthesis in the genus Lactobacillus . Using efficient screening techniques, extracellular glucosyltransferase (GTF) enzyme activity, resulting in alpha-glucan synthesis from sucrose, was detected in various lactobacilli. PCR with degenerate primers based on homologous boxes of known glucosyltransferase (gtf ) genes of lactic acid bacteria strains allowed cloning of fragments of 10 putative gtf genes from eight different glucan producing Lactobacillus strains (five Lactobacillus reuteri strains, one Lactobacillus fermentum strain , one Lactobacillus sake strain and one Lactobacillus parabuchneri strain) ....
Subjects
Medical Subject Headings: food and beveragesstomatognathic diseasesstomatognathic system
free text keywords: Nutrition, Food technology, Glucan, Glucansucrase, Glucosyltransferase, Lactobacillus, Polysacch aride, Sucrose, Bacteria, Chemical bonds, Genes, Sugar (sucrose), Synthesis (chemical), Screening techniques, Enzymes, lactic acid, polysaccharide, bacterial strain, carbohydrate synthesis, cloning, conference paper, controlled study, enzyme activity, gene identification, genetic variability, glycosidation, Lactobacillus fermentum, Lactobacillus reuteri, Lactobacillus sakei, Leuconostoc, nonhuman, nucleotide sequence, polymerase chain reaction, screening test, sequence analysis, Streptococcus, Bacteria (microorganisms), Lactobacillus parabuchneri, Prokaryota, STREPTOCOCCUS-SALIVARIUS ATCC-25975, MESENTEROIDES NRRL B-512F, MOLECULAR CHARACTERIZATION, SEQUENCE-ANALYSIS, NUCLEOTIDE-SEQUENCE, DEXTRANSUCRASE, EXOPOLYSACCHARIDES, ALPHA(1-6), Biotechnology, Biochemistry, Catalysis, biology.organism_classification, biology, chemistry.chemical_classification, chemistry, Microbiology, biology.protein
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