Efficient screening methods for glucosyltransferase genes in Lactobacillus strains

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Kralj, S.; Geel van - Schutten, G.H.; Maarel, M.J.E.C. van der; Dijkhuizen, L.;
(2003)
  • Subject: glucansucrase | polysaccharide | STREPTOCOCCUS-SALIVARIUS ATCC-25975 | NUCLEOTIDE-SEQUENCE | MESENTEROIDES NRRL B-512F | MOLECULAR CHARACTERIZATION | GLUCAN | sucrose | glucosyltransferase | SEQUENCE-ANALYSIS | ALPHA(1-6) | Lactobacillus | EXOPOLYSACCHARIDES | DEXTRANSUCRASE | CLONING
    mesheuropmc: food and beverages | stomatognathic diseases | stomatognathic system

Limited information is available about homopolysaccharide synthesis in the genus Lactobacillus . Using efficient screening techniques, extracellular glucosyltransferase (GTF) enzyme activity, resulting in alpha-glucan synthesis from sucrose, was detected in various lact... View more
  • References (35)
    35 references, page 1 of 4

    Arguello-Morales, M.A., Remaud-Simeon, M., Pizzut, S., Sarcabal, P. and Monsan, P. (2000) ''Sequence analysis of the gene encoding alternansucrase, a sucrose glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355'', FEMS Microbiol. Lett. 182, 81 /85.

    Ausubel, F.M., Brent, R.E., Kingston, D.D., Moore, J.G., Seidman, J.G., Smith, J.A. and Struhl, K. (1987) Current Protocols in Molecular Biology (John Wiley & sons, New York).

    Bozonnet, S., Dols-Laffargue, M., Fabre, E., Pizzut, S., RemaudSimeon, M., Monsan, P. and Willemot, R.M. (2002) ''Molecular characterization of DSR-E, an alpha-1,2 linkage-synthesizing dextransucrase with two catalytic domains'', J Bacteriol. 184, 5753 /5761.

    De Man, J.C., Rogosa, M. and Sharpe, M.E. (1960) ''A medium for the cultivation of lactobacilli'', J. Appl. Bacteriol. 23, 130 /135.

    Dunican, L.K. and Seeley Jr., H.W. (1963) ''Temperature-sensitive dextransucrase production by a Lactobacillus '', J. Bacteriol. 86, 1097 /1083.

    Ferretti, J.J., Gilpin, M.L. and Russell, R.R. (1987) ''Nucleotide sequence of a glucosyltransferase gene from Streptococcus sobrinus MFe28'', J. Bacteriol. 169, 4271 /4278.

    Funane, K., Mizuno, K., Takahara, H. and Kobayashi, M. (2000) ''Gene encoding a dextransucrase-like protein in Leuconostoc mesenteroides NRRL B-512F'', Biosci. Biotechnol. Biochem. 64, 29 /38.

    Giffard, P.M., Allen, D.M., Milward, C.P., Simpson, C.L. and Jacques, N.A. (1993) ''Sequence of the gtfK gene of Streptococcus salivarius ATCC 25975 and evolution of the gtf genes of oral streptococci'', J. Gen. Microbiol. 139, 1511 /1522.

    Giffard, P.M., Simpson, C.L., Milward, C.P. and Jacques, N.A. (1991) ''Molecular characterization of a cluster of at least two glucosyltransferase genes in Streptococcus salivarius ATCC 25975'', J. Gen. Microbiol. 137, 2577 /2593.

    Gilmore, K.S., Russell, R.R. and Ferretti, J.J. (1990) ''Analysis of the Streptococcus downei gtfS gene, which specifies a glucosyltransferase that synthesizes soluble glucans'', Infect. Immun. 58, 2452 /2458.

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