publication . Article . Other literature type . 2006

Altered balance of glutamatergic/GABAergic synaptic input and associated changes in dendrite morphology after BDNF expression in BDNF-deficient hippocampal neurons.

María Ángeles Arévalo; Jochen C. Meier; Rosemarie Grantyn; B. Singh; Alfredo Rodríguez-Tébar; Christian Henneberger; D. Betances;
Open Access
  • Published: 05 Jul 2006 Journal: Journal of Neuroscience, volume 26, pages 7,189-7,200 (issn: 0270-6474, eissn: 1529-2401, Copyright policy)
  • Publisher: Society for Neuroscience
  • Country: Spain
Abstract
Cultured neurons from bdnf-/- mice display reduced densities of synaptic terminals, although in vivo these deficits are small or absent. Here we aimed at clarifying the local responses to postsynaptic brain-derived neurotrophic factor (BDNF). To this end, solitary enhanced green fluorescent protein (EGFP)-labeled hippocampal neurons from bdnf-/- mice were compared with bdnf-/- neurons after transfection with BDNF, bdnf-/- neurons after transient exposure to exogenous BDNF, and bdnf+/+ neurons in wild-type cultures. Synapse development was evaluated on the basis of presynaptic immunofluorescence and whole-cell patch-clamp recording of miniature postsynaptic curre...
Subjects
Medical Subject Headings: nervous system
free text keywords: General Neuroscience, synaptogenesis, dendrite growth, E/I balance, bdnf knock-out, neuron transfection, retrograde messenger., Function and Dysfunction of the Nervous System, 570 Life Sciences, 610 Medical Sciences, Medicine, Dendrite growth, E/I balance, bdnf knock-out, Neuron transfection, Retrograde messenger, Animals, Mice, Articles, Postsynaptic potential, Synapse, Tropomyosin receptor kinase B, Glutamate receptor activity, Biology, Glutamatergic, Dendritic spine, Synaptogenesis, Dendrite, medicine.anatomical_structure, medicine, Neuroscience
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