Cloning of the Gene Encoding a Novel Lantibiotic Nukacin ISK-1, of Staphylococcus warneri

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Sashihara, Toshihiro; Kimura, Hirokazu; Higuchi, Toshimasa; Adachi, Asaho; Matsusaki, Hiromi; Sonomoto, Kenji; Ishizaki, Ayaaki;
  • Publisher: Kyushu University
  • Journal: 九州大学大学院農学研究院紀要, volume 45, issue 1, pages 149-161 (issn: 0023-6152)
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  • Subject: Open reading frame (ORF) | ISK-1 | Single-specific-primer-PCR (SSP-PCR) | Cloning

Staphylococcus warneri ISK-1, we had reported as Pediococcus sp. ISK-1 previously, produces a novel bacteriocin, nukacin ISK-1. Edman degradation of the chemically reduced nukacin ISK-1 revealed a sequence of 27 amino acids, 7 of which were unidentified. Using single-sp... View more
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