publication . Article . 2017

SRSF1-3 contributes to diversification of the immunoglobulin variable region gene by promoting accumulation of AID in the nucleus

Yuka Kawaguchi; Hiroaki Nariki; Naoko Kawamoto; Yuichi Kanehiro; Satoshi Miyazaki; Mari Suzuki; Masaki Magari; Hiroshi Tokumitsu; Naoki Kanayama;
Open Access English
  • Published: 01 Apr 2017 Journal: Biochemical and Biophysical Research Communications, volume 485, issue 2, pages 261-266 (issn: 0006-291X, Copyright policy)
  • Publisher: Elsevier
Abstract
Activation-induced cytidine deaminase (AID) is essential for diversification of the Ig variable region (IgV). AID is excluded from the nucleus, where it normally functions. However, the molecular mechanisms responsible for regulating AID localization remain to be elucidated. The SR-protein splicing factor SRSF1 is a nucleocytoplasmic shuttling protein, a splicing isoform of which called SRSF1-3, has previously been shown to contribute to IgV diversification in chicken DT40 cells. In this study, we examined whether SRSF1-3 functions in IgV diversification by promoting nuclear localization of AID. AID expressed alone was localized predominantly in the cytoplasm. I...
Subjects
free text keywords: AID, DT40, Gene conversion, Ig, SRSF1, Somatic hypermutation, Splicing factor, Genetics, Nuclear protein, Cytidine deaminase, Biology, Nuclear localization sequence, Nuclear transport, RNA splicing, Mutant
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publication . Article . 2017

SRSF1-3 contributes to diversification of the immunoglobulin variable region gene by promoting accumulation of AID in the nucleus

Yuka Kawaguchi; Hiroaki Nariki; Naoko Kawamoto; Yuichi Kanehiro; Satoshi Miyazaki; Mari Suzuki; Masaki Magari; Hiroshi Tokumitsu; Naoki Kanayama;