publication . Article . 2005

Characterization of phospholipase A2 from the pyloric ceca of two species of starfish, Coscinasterias acutispina and Plazaster borealis

Kishimura, Hideki; Hayashi, Kenji;
Open Access
  • Published: 01 Sep 2005 Journal: Food Chemistry, volume 92, pages 407-411 (issn: 0308-8146, Copyright policy)
  • Publisher: Elsevier BV
Abstract
Abstract Phospholipase A (PLA) activities in the pyloric ceca and viscera from seven species of marine invertebrates (four starfish, one sea urchin, and two shellfish) were determined. Relatively high PLA specific activities were found in the pyloric ceca of two species of starfish ( Coscinasterias acutispina and Plazaster borealis ). Phospholipase A 2 s (PLA 2 s) were partially purified from the pyloric ceca of the starfish, C. acutispina PLA 2 (C-PLA 2 ) and P. borealis PLA 2 (P-PLA 2 ). The C-PLA 2 and P-PLA 2 mainly released oleic acid from 1-palmitoyl-2-oleoyl- sn -glycero-3-phosphocholine. Temperature optima of the C-PLA 2 and P-PLA 2 were at around 60 °C ...
Subjects
free text keywords: Food Science, Analytical Chemistry, General Medicine, Sea urchin, biology.animal, biology, Biochemistry, Oleic acid, chemistry.chemical_compound, chemistry, Phospholipase A2, biology.protein, Phosphatidylethanolamine, Fatty acid, chemistry.chemical_classification, Phospholipase A, Phosphatidylcholine, Starfish, biology.organism_classification, Coscinasterias acutispina, Plazaster borealis, Marine invertebrate, Pyloric cecum, Polar group specificity
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publication . Article . 2005

Characterization of phospholipase A2 from the pyloric ceca of two species of starfish, Coscinasterias acutispina and Plazaster borealis

Kishimura, Hideki; Hayashi, Kenji;