Alkaline Extraction of DNA from Pathogenic Fungi for PCR-RFLP Analysis

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Matsumoto, Masaru; Mishima, Shinobu; Matsuyama, Nobuaki; 松元, 賢; 松山, 宣明;
(1997)
  • Publisher: Kyushu University
  • Journal: 九州大学大学院農学研究院紀要,volume 42,issue 1/2,pages23-26 (issn: 0023-6152)
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  • Subject: PCR-RFLP analysis | Pathogenic fungi | Alkaline extraction
    mesheuropmc: food and beverages

For the preparation of DNA samples from fungal mycelia alkaline extraction method was applied and assessed its usefulness for PCR-RFLP analysis. Using alkaline treatment protocols, 18S ribosomal DNAs (rDNA) derived from fungal genomic DNA of Pyricularia oryzae, P. zingi... View more
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