publication . Research . 1997

Alkaline Extraction of DNA from Pathogenic Fungi for PCR-RFLP Analysis

Matsumoto, Masaru; Mishima, Shinobu; Matsuyama, Nobuaki; 松元, 賢; 松山, 宣明;
Open Access English
  • Published: 01 Dec 1997 Journal: 九州大学大学院農学研究院紀要, volume 42, issue 1/2, pages 23-26 (issn: 0023-6152, Copyright policy)
  • Publisher: Kyushu University
Abstract
For the preparation of DNA samples from fungal mycelia alkaline extraction method was applied and assessed its usefulness for PCR-RFLP analysis. Using alkaline treatment protocols, 18S ribosomal DNAs (rDNA) derived from fungal genomic DNA of Pyricularia oryzae, P. zingiberi, Rhizoctonia solani and R. oryzae were PCR-amplified and digested with Hha I, Msp I and Hae ill. RFLP analysis with HhaI showed the divergent polymorphism between genus Pyricularia and Rhizoctonia. The alkaline DNA extraction method saves the time and labor required for DNA sample preparation and seemed to be useful for rapid identification of specific fungal species by PCR-RFLP analysis.
Subjects
mesheuropmc: food and beverages
free text keywords: PCR-RFLP analysis, Pathogenic fungi, Alkaline extraction
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Research . 1997
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