Catalytically important amino-acid residues of abalone alginate lyase HdAly assessed by site-directed mutagenesis

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Yamamoto, Sayo; Sahara, Takehiko; Sato, Daisuke; Kawasaki, Kosei; Ohgiya, Satoru; Inoue, Akira; Ojima, Takao;
(2008)
  • Publisher: Elsevier
  • Journal: Enzyme and Microbial Technology,volume 43,issue 6,pages396-402 (issn: 0141-0229)
  • Subject: alginate lyase | abalone | Haliotis discus hannai | cold-inducible expression in yeast | recombinant enzyme | site-directed mutagenesis

Alginate lyase is an enzyme that degrades alginate chains via β-elimination and has been used for the production of alginate oligosaccharides and protoplasts from brown algae. Previously, we deduced the amino-acid sequence of an abalone alginate lyase, HdAly, from its c... View more
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