Catalytically important amino-acid residues of abalone alginate lyase HdAly assessed by site-directed mutagenesis
- Publisher: Elsevier
Enzyme and Microbial Technology,
alginate lyase | abalone | Haliotis discus hannai | cold-inducible expression in yeast | recombinant enzyme | site-directed mutagenesis
Alginate lyase is an enzyme that degrades alginate chains via β-elimination and has been used for the production of alginate oligosaccharides and protoplasts from brown algae. Previously, we deduced the amino-acid sequence of an abalone alginate lyase, HdAly, from its c...