publication . Article . 2013

Las proteínas del plasma seminal incrementan la viabilidad espermática post-descongelación del semen de toros Sanmartinero

Rocío Herrera L.; Luis Arbeláez R.; Jaime Cardoso C.; Jaime Cardoso C.; Miguel Peña J.; Miguel Peña J.; Henry Velásquez P.; Henry Velásquez P.; Fabián Rueda A.; Fabián Rueda A.; ...
Open Access English
  • Published: 12 Jan 2013 Journal: Revista MVZ Cordoba (issn: 0122-0268, eissn: 1909-0544, Copyright policy)
  • Publisher: Universidad de Cordoba
Abstract
Objetivo. El objetivo de este trabajo fue evaluar el efecto de la adición de proteínas del plasma seminal sobre el porcentaje de espermatozoides bovinos viables post-descongelación. Materiales y métodos. Los espermatozoides se congelaron usando dos medios (citrato-fructosa-yema y Bioxcell®) y la obtención de proteínas de plasma seminal de bajo peso molecular se realizó por medio de cromatografía líquida de baja presión. Las proteínas de interés eluyeron en las fracciones 21-25 y se sometieron a electroforésis en una y dos dimensiones. Los espermatozoides se incubaron a 37°C durante una hora, con 0.5, 1.0, 1.5 y 2.0 mg de la fracción 21-25. Se incluyeron dos trat...
Subjects
free text keywords: Ciencias Agrícolas, Ciencias Veterinarias, Capacitación espermática, criopreservación, espermatozoide, plasma seminal, proteínas de choque térmico, Veterinary medicine, SF600-1100, heat shock proteins, seminal plasma, sperm capacitation, Cryopreservation; heat shock proteins; seminal plasma; sperm capacitation; sperm, Animal Science and Zoology, Aquatic Science, General Veterinary, Electrophoresis, Incubation, Seminal Plasma Proteins, Endocrinology, medicine.medical_specialty, medicine, Internal medicine, Sperm cryopreservation, Sperm, Cryopreservation, Biology, Heat shock protein, Andrology, Semen
24 references, page 1 of 2

1. Barrios B, Perez PE, Gallego M. Seminal plasma proteins revert the cold-shock damage on ram sperm membrane. Biol Reprod 2000; 63:1531-1537.

2. Moura A, Hasan K. Identification of proteins in accessory sex gland fluid associated with fertility indexes of dairy bulls. J Androl 2006; 27(2):201-212.

3. Watson PF. Cooling of spermatozoa and fertility capacity. Reprod Domest Anim 1999; 31:135-40.

4. Moussa M, Matinet V, Trimeche A, Tainturier D, Anton M. Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozenthawed bull semen. Theriogenology 2002; 57:1695-706. [OpenAIRE]

5. Gil J, Rodiguez-Irazoqui M, Lundeheim N, Soderquist L, Rodriguez-Martinez H. Fertility of ram semen frozen in Bioexcell and used for cervical artificial insemination. Theriogenology 2003; 59:1157-70.

6. Forouzanfar M, Sharafi SM, Hosseini S, Ostadhosseini M, Hajian L, Hosseini P et al. In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology 2010; 73:480-487. [OpenAIRE]

7. Bousseau S, Brillard JP, Guienne M, Guerin B, Camus A, Lechat M. Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin-based diluents. Theriogenology 1998; 50:699-706. [OpenAIRE]

8. Gwathmey T M. PDC-109 (BSP-A1/A2) promotes bull sperm binding to oviductal epithelium in vitro and may be involved in forming the oviductal sperm reservoir. Biol Reprod 2003; 69:809-815.

9. Manjunath P, Lefevbre J, Wright W. 2009. New nomenclature for mammalian BSP genes. Biol Reprod 2009; 80:394-397. [OpenAIRE]

10. Barrios B, Fernández-Juan M, Muiño-Blanco T, Cebrián-Pérez J.A. Immunocytochemical localization and biochemical characterization of two seminal plasma proteins which protect ram spermatozoa against coldshock. J Androl 2005; 26:539-549.

11. Desnoyers L, Therien I, Manjunath, P. Characterization of the major proteins of bovine seminal fluid by two dimensional polyacrilamide gel electrophoresis. Mol Reprod Dev 1994; 37:425-435.

12. Cross NL. Multiple effects of seminal plasma on the acrosome reaction of human sperm. Mol Reprod Dev 1993; 35:316-323.

13. Fernández-Juan M, Gallego M, Barrios B, Osada J, Cebrián-Pérez J.A, Muiño-Blanco T. Immunohistochemical localization of spermpreserving proteins in the ram reproductive tract. J Androl 2006; 27:588-595.

14. Perez-Pe R, Cebrián-Perez J, Muiño-Blanco T. Semen plasma proteins prevent cold-shock membrane damage to ram spermatozoa. Theriogenology 2000; 56:425-434.

15. Colas C, Junquera C, Perez-Pe R, CebriánPérez JA, Muiño-Blanco T. Ultrastructural study of the ability of semnial plasma proteins to protect ram spermatozoa against cold-shock. Microsc Res Tech 2009; 72:566-572. [OpenAIRE]

24 references, page 1 of 2
Powered by OpenAIRE Open Research Graph
Any information missing or wrong?Report an Issue
publication . Article . 2013

Las proteínas del plasma seminal incrementan la viabilidad espermática post-descongelación del semen de toros Sanmartinero

Rocío Herrera L.; Luis Arbeláez R.; Jaime Cardoso C.; Jaime Cardoso C.; Miguel Peña J.; Miguel Peña J.; Henry Velásquez P.; Henry Velásquez P.; Fabián Rueda A.; Fabián Rueda A.; ...