Association between HLA-A, HLA-C and HLA-B Genes and Ankylosing Spondylitis in Macedonian Population

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Kirijas, Meri ; Mishevska-Perchinkova, Snezhana ; Karadzova-Stojanoska, Anzelika ; Efinska-Mladenovska, Olivija ; Petlichkovski, Aleksandar ; Trajkov, Dejan ; Spiroski, Mirko (2014)
  • Publisher: ID Design 2012/DOOEL Skopje, Republic of Macedonia
  • Journal: Macedonian Journal of Medical Sciences (issn: 1857-5773, eissn: 1857-5749)
  • Subject: HLA class I; HLA-B*27; ankylosing spondylitis; disease association; Republic of Macedonia

Aim: The aim of this study was to determine the association of HLA-A, -C and -B genes with ankylosing spondylitis in patients from the Republic of Macedonia.Material and Methods: This study included 307 subjects (250 healthy individuals and 57 patients with ankylosing spondylitis who were diagnosed at the University Clinic of Rheumatology in Skopje). The HLA typing of class 1 (HLA-A, HLA-C and HLA-B) genes was performed using the method of Reverse Line Strip, after isolation of DNK from the blood leucocytes with the standard phenol-chloroforme method. The HLA sub typing of HLA-B*27 was performed with high resolution single-strand polymorphism.Results: We found negative association between HLA-A*03, HLA-A*24, HLA-C*04, and HLA-B*18, as well as positive association between HLA-C*01, HLA-C*02, and HLA-B*27 group specific alleles and patients with ankylosing spondylitis. Both HLA-B*27:02 and HLA-B*27:05 alleles have shown positive association with ankylosing spondylitis.Conclusion: Group specific alleles HLA-A*03, HLA-A*24, HLA-C*04, and HLA-B*18 are significantly more frequent in healthy individuals than in patients with ankylosing spondylitis which suggests their protective role. HLA-C*01, HLA-C*02, and HLA-B*27 group specific alleles (HLA-B*27:02 and HLA-B*27:05) have strong positive or susceptible association with ankylosing spondylitis.
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