publication . Article . 2012

Detection of Shiga toxin-producing Escherichia coli by sandwich enzyme-linked immunosorbent assay using chicken egg yolk IgY antibodies

Parma, Y. R.; Chacana, P. A.; Lucchesi, P. M.; Rogé, A.; Granobles Velandia, C. V.; Alejandra Krüger; Parma, A. E.; Fernández-Miyakawa, M. E.;
Open Access English
  • Published: 01 Jun 2012 Journal: Frontiers in Cellular and Infection Microbiology (issn: 2235-2988, Copyright policy)
  • Publisher: Frontiers Media S.A.
Abstract
Enterohemorrhagic Escherichia coli (EHEC), a subset of Shiga toxin producing E. coli (STEC) is associated with a spectrum of diseases that includes diarrhea, hemorrhagic colitis and a life-threatening hemolytic uremic syndrome (HUS). Regardless of serotype, Shiga toxins (Stx1 and/or Stx2) are uniformly expressed by all EHEC, and so exploitable targets for laboratory diagnosis of these pathogens. In this study, a sandwich ELISA for determination of Shiga toxin (Stx) was developed using anti-Stx2 B subunit antibodies and its performance was compared with that of the Vero cell assay and a commercial immunoassay kit. Chicken IgY was used as capture antibody and a HR...
Subjects
free text keywords: Egg Yolk, Escherichia coli, Shiga Toxin, STEC, ELISA, IgY, Microbiology, QR1-502, Original Research Article, chicken, Immunology, Microbiology (medical), Infectious Diseases, medicine.disease_cause, medicine, Vero cell, Antibody, biology.protein, biology, Virology, Serotype, STX2, Immunoassay, medicine.diagnostic_test, Recombinant DNA, law.invention, law
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