Izolacija protoplasta iz pupova Picea abies

Cesar-Lepeduš, Vera

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Izolacija protoplasta iz pupova Picea abies

descriptionPublicationkeyboard_double_arrow_right Conference object 01 Jan 1986

Authors: Cesar-Lepeduš, Vera;

Izolacija protoplasta iz pupova Picea abies

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Abstract

O izolaciji protoplasta četinjača nije objavljeno mnogo radova. U predstavljenom eksperimentu postupak izolacije protoplasts iz pupova vrste Picea abies uključivao je sakupljanje, površinsku sterilizaciju i izolaciju dostatnog broja pupova da bi se imalo dovoljno svježe tvari za enzimski tretman. Postupak izolacije protoplasta ponavljan je četiri puta. Prva i četvrta izolacija rezultirale su akseničnom kulturom protoplasta koji su se dijelili i održali u životu više od šest tjedana.

Keywords

protoplasts, Picea abies, vegetative buds, vegetativni pupovi, protoplasti

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