publication . Article . 2009

Differential utilization of blood meal amino acids in mosquitoes

Roger Miesfeld;
Open Access English
  • Published: 16 Sep 2009 Journal: Open Access Insect Physiology (issn: 1179-2787, Copyright policy)
  • Publisher: Dove Press
Abstract
Guoli Zhou, Roger MiesfeldDepartment of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, USAAbstract: Amino acids in the mosquito blood meal have two forms, protein-bound and plasma-free amino acids. To determine if the metabolic fate and flux of these two forms of blood meal amino acids are distinct, we fed mosquitoes eight [14C]-labeled amino acids, seven of which are essential for mosquitoes (leucine, valine, isoleucine, phenylalanine, lysine, arginine, histidine), and one that can be readily converted to metabolic fuel (alanine). These 14C tracer amino acids were either incorporated into an in vitro synthesized protein (protein-bound) and fed w...
Subjects
free text keywords: Open Access Insect Physiology, Amino acid, chemistry.chemical_classification, chemistry, Biology, Biochemistry, Blood meal, Egg protein, Phenylalanine, Alanine, Valine, Isoleucine, Leucine
Related Organizations
Funded by
NIH| POSTTRANSCRIPTIONAL GENE REGULATION IN T CELL ACTIVATION
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5R01AI046451-03
  • Funding stream: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
41 references, page 1 of 3

1. Zhou G, Flowers M, Friedrich K, Horton J, Pennington J, Wells MA. Metabolic fate of [14C]-labeled meal protein amino acids in Aedes aegypti mosquitoes. J Insect Physiol. 2004;50(4):337-349.

2. Adibi SA. Influence of dietary deprivations on plasma concentration of free amino acids of man. J Applied Physiol. 1968;25(1):52-57.

3. Noriega FG, Colonna AE, Wells MA. Increase in the size of the amino acid pool is sufficient to activate translation of early trypsin mRNA in Aedes aegypti midgut. Insect Biochem Mol Biol. 1999;29(3):243-247.

4. BrandonMC,PenningtonJE,IsoeJ,ZamoraJ,SchillingerAS,MiesfeldRL. TOR signaling is required for amino acid stimulation of early trypsin protein synthesis in the midgut of Aedes aegypti mosquitoes. Insect Biochem Mol Biol. 2008;38(10):916-922.

5. Caroci AS, Noriega FG. Free amino acids are important for the retention of protein and non-protein meals by the midgut of Aedes aegypti females. J Insect Physiol. 2003;49(9):839-844.

6. Uchida K, Oda T, Matsuoka H, et al. Induction of oogenesis in mosquitoes (Diptera: Culicidae) by infusion of the hemocoel with amino acids. J Med Ent. 2001;38(4):572-575.

7. Greenberg J. Some nutritional requirements of adult mosquitoes (Aedes aegypti) for oviposition. J Nutr. 1951;43(1):27-35. [OpenAIRE]

8. Lea AO, Dimond JB, Delong DM. Role of diet in egg development by mosquitoes (Aedes aegypti). Science. 1956;123(3203):890-891. [OpenAIRE]

9. Chang YH, Judson CL. The role of isoleucine in differential egg production by the mosquito Aedes aegypti Linnaeus (Diptera: Culicidae) following feeding on human or guinea pig blood. Comp Biochem Physiol. 1977;57(1 Pt A):23-28.

10. Chang YH, Judson CL. Amino acid composition of human and guinea pig blood proteins and ovarian proteins of the yellow fever mosquito Aedes aegypti; and their effects on the mosquito egg production. Comp Biochem Physiol. 1979;62(3 Pt A):753-755.

11. Harrington LC, Edman JD, Scott TW. Why do female Aedes aegypti (Diptera: Culicidae) feed preferentially and frequently on human blood? J Med Ent. 2001;38(3):411-422.

12. Penna TC, Chiarini E, Machoshvili IA, Ishii M, Pessoa A. Intracellular release of recombinant green fluorescent protein (gfp(uv)) from Escherichia coli. Applied Biochem Biotech. 2002;(98-100):791-802.

13. Richards HA, Han C, Hopkins RG, Failla ML, Ward WW, Stewart CN. Safety assessment of recombinant green fluorescent protein orally administered to weaned rats. J Nutr. 2003;133(6):1909-1912.

14. Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ. Primary structure of the Aequorea victoria green-fluorescent protein. Gene. 1992;111(2):229-233. [OpenAIRE]

15. Inouye S, Tsuji FI. Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein. FEBS Lett. 1994;341(2-3):277-280.

41 references, page 1 of 3
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publication . Article . 2009

Differential utilization of blood meal amino acids in mosquitoes

Roger Miesfeld;