publication . Article . 2006

Comparative evaluation of the Ridascreen® Verotoxin enzyme immunoassay for detection of Shiga‐toxin producing strains of Escherichia coli (STEC) from food and other sources

L. Beutin; H. Steinrück; G. Krause; K. Steege; S. Haby; G. Hultsch; B. Appel;
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  • Published: 30 Aug 2006 Journal: Journal of Applied Microbiology, volume 102, pages 630-639 (issn: 1364-5072, eissn: 1365-2672, Copyright policy)
  • Publisher: Wiley
Abstract
AIMS: To evaluate the suitability of the commercially distributed Ridascreen Verotoxin enzyme immunoassay (EIA) for detection of known genetic types of the Vero (Shiga) toxins 1 (Stx1) and 2 (Stx2) families and to determine its relative sensitivity and specificity. METHODS AND RESULTS: The Ridascreen-EIA was compared with the Vero cell assay, a P(1)-glycoprotein receptor EIA and with stx gene-specific PCs for detection of Stx with 43 Shiga toxin-producing strains of Escherichia coli (STEC) reference strains and with 241 test strains. The Ridascreen-EIA detects strains producing Stx1 and variants Stx1c and Stx1d, as well as Stx2 and variants Stx2d1, Stx2d2, Stx2e...
Subjects
Medical Subject Headings: fluids and secretionshemic and lymphatic diseases
free text keywords: Biotechnology, Applied Microbiology and Biotechnology, General Medicine
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