publication . Article . 1995

quantitative analysis of comparative genomic hybridization

Stanislas du Manoir; Evelin Schröck; Martin Bentz; Michael R. Speicher; Stefan Joos; Thomas Ried; Peter Lichter; Thomas Cremer;
  • Published: 01 Jan 1995 Journal: Cytometry, volume 19, pages 27-41 (issn: 0196-4763, eissn: 1097-0320, Copyright policy)
  • Publisher: Wiley
Comparative genomic hybridization (CGH) is a new molecular cytogenetic method for the detection of chromosomal imbalances. Following cohybridization of DNA prepared from a sample to be studied and control DNA to normal metaphase spreads, probes are detected via different fluorochromes. The ratio of the test and control fluorescence intensities along a chromosome reflects the relative copy number of segments of a chromosome in the test genome. Quantitative evaluation of CGH experiments is required for the determination of low copy changes, e.g., monosomy or trisomy, and for the definition of the breakpoints involved in unbalanced rearrangements. In this study, a ...
free text keywords: Biophysics, Pathology and Forensic Medicine, Cell Biology, Endocrinology, Hematology, Breakpoint, Fluorescence in situ hybridization, medicine.diagnostic_test, medicine, Comparative genomic hybridization, Molecular biology, Monosomy, medicine.disease, DNA–DNA hybridization, Metaphase, Biology, Chromosome, Trisomy
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